Information on EC 2.4.1.57 - phosphatidylinositol alpha-mannosyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.57
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RECOMMENDED NAME
GeneOntology No.
phosphatidylinositol alpha-mannosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
transfers one or more alpha-D-mannose residues from GDP-mannose to positions 2,6 and others in 1-phosphatidyl-myo-inositol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:1-phosphatidyl-1D-myo-inositol alpha-D-mannosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37277-65-9
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-alpha-D-mannopyranose + 1-hexadecanoyl-3-(2-(6-O-hexadecanoyl-beta-D-mannopyranosyl)-1D-myo-inositolphospho)-2-nonadecanoyl-sn-glycerol
GDP + 1-hexadecanoyl-3-(2-(6-O-hexadecanoyl-beta-D-mannopyranosyl)-6-O-alpha-D-mannopyranosyl-1D-myo-inositolphospho)-2-nonadecanoyl-sn-glycerol
show the reaction diagram
-
reaction catalyzed by PimB
-
?
GDP-alpha-D-mannopyranose + 1-hexadecanoyl-3-(2-(6-O-hexadecanoyl-beta-D-mannopyranosyl)-6-O-alpha-D-mannopyranosyl-1D-myo-inositolphospho)-2-nonadecanoyl-sn-glycerol
GDP + 1-hexadecanoyl-3-(2-(6-O-hexadecanoyl-beta-D-mannopyranosyl)-6-(6-0-alpha-D-mannopyranosyl-alpha-D-mannopyranosyl)-1D-myo-inositolphospho)-2-nonadecanoyl-sn-glycerol
show the reaction diagram
-
reaction catalyzed by PimC
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?
GDP-alpha-D-mannopyranose + phosphatidyl-myo-inositol
GDP + ?
show the reaction diagram
-
attaches mannosyl selectively to the 6-OH of the inositol moiety
-
-
?
GDP-Man + phosphatidyl-myo-inositol
?
show the reaction diagram
assay at pH 7.5, 37C, reaction stopped with CHCl3/CH3OH
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-
?
GDP-mannose + 1,2-dioctanoyl-sn-glycero-3-phosphoinositol
?
show the reaction diagram
-
-
-
-
?
GDP-mannose + 1-phosphatidyl-myo-inositol
?
show the reaction diagram
-
-
-
-
?
GDP-mannose + phosphatidyl-myo-inositol
GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
show the reaction diagram
GDP-mannose + phosphatidylinositol
GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
show the reaction diagram
GDP-mannose + phosphatidylinositol
GDP + phosphatidyl-(2-O-alpha-D-mannopyranosyl)-myo-inositol
show the reaction diagram
-
-
-
-
?
GDPmannose + phosphatidyl-myo-inositol
GDP + phosphatidyl-myo-inositol monomannoside
show the reaction diagram
-
-
-
?
UDP-mannose + 3-deoxy-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)-3-deoxy-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + 4-deoxy-4-amino-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)-4-deoxy-4-amino-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + 4-deoxy-4-fluoro-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)-4-deoxy-4-fluoro-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + 4-deoxy-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)-4-deoxy-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + 6-deoxy-4-fluoro-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)-6-deoxy-4-fluoro-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + 6-deoxy-6-amino-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)-6-deoxy-6-amino-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + 6-deoxy-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)-6-deoxy-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + Manalpha(1-6)-2-deoxy-Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)Manalpha(1-6)-2-deoxy-Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + Manalpha(1-6)-3-deoxy-Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)Manalpha(1-6)-3-deoxy-Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
UDP-mannose + Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
UDP + Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
show the reaction diagram
-
assay at 30C, 15 min
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-mannose + 1-phosphatidyl-myo-inositol
?
show the reaction diagram
-
-
-
-
?
GDP-mannose + phosphatidyl-myo-inositol
GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
show the reaction diagram
GDP-mannose + phosphatidylinositol
GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
show the reaction diagram
additional information
?
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essential enzyme for mycobacterial growth
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-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-deoxy-2-amino-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
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2-deoxy-2-fluoro-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
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3-deoxy-3-amino-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
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3-deoxy-3-fluoro-Manalpha(1-6)Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
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Manalpha(1-6)-4-deoxy-Manalpha(1-4)GlcNalpha(1-6)-D-myo-inositol-1-octadecyl phosphate
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methyl 6-deoxy-6-dihydroxyphosphinyl-beta-D-galactopyranoside
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
isopropyl-1-thio-beta-D-galactopyranoside
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additional information
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PimA attaches to the membrane through its N-terminal domain and this association leads to enzyme activation
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
-
predicted from cDNA
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
PEG 3350, pH 5.5
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crystal structures of PimA in complex with GDP and GDP-Man are determined using multiplewavelength anomalous diffraction methods at 2.4 A and 2.6 A resolution respectively; crystal structures of PimA in complex with GDP and GDP-Man is determined using multiple-wavelength anomalous diffraction methods at 2.4 and 2.6 A of resolution, respectively
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PimA-GDP-Manp complex
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sitting-drop vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A and diffract to 2.4 A resolution
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
GDP-Man stabilizes PimA, phosphatidyl-myo-inositol destabilizes PimA after formation of a more relaxed complex
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
combination of metal ion affinity and anionic exchange and gel filtration chromatography
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immobilized metal ion affinity chromatography (Ni2+)
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Talon resin column chromatpgraphy and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
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expressed in Escherichia coli BL3 cells
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expression in Escherichia coli
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expression in Escherichia coli BL21
expression in Escherichia coli BL21(DE3)pLysS cells transformed wth pET-pimA
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expression in Myobacterium smegmatis; overexpression in Escherichia coli
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the pimA gene is cloned into the pET82a vector and the recombinant plasmid is transformed into Escherichia coli BL21 (DE3)
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is downregulated by the addition of anhydrotetracycline to the culture medium
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D13A
-
1.9% of wild type activity (blank has 0.5% activity)
D13N
-
1.5% of wild type activity (blank has 0.5% activity)
D13Y
-
0.3% of wild type activity (blank has 0.5% activity)
E290D
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2.9% of wild type activity (blank has 0.5% activity)
E290N
-
1.1% of wild type activity (blank has 0.5% activity)
E290Q
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4.6% of wild type activity (blank has 0.5% activity)
G123P
-
86.6% of wild type activity (blank has 0.5% activity)
G20W
-
3.2% of wild type activity (blank has 0.5% activity)
G291S
-
5.3% of wild type activity (blank has 0.5% activity)
H118S
-
0.7% of wild type activity (blank has 0.5% activity)
H120S
-
81.2% of wild type activity (blank has 0.5% activity)
I21A
-
2.0% of wild type activity (blank has 0.5% activity)
I21S
-
2.4% of wild type activity (blank has 0.5% activity)
K211Q
-
3.5% of wild type activity (blank has 0.5% activity)
N12A
-
31.2% of wild type activity (blank has 0.5% activity)
Q22A
-
14.3% of wild type activity (blank has 0.5% activity)
R206S
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5.6% of wild type activity (blank has 0.5% activity)
R210S
-
8.7% of wild type activity (blank has 0.5% activity)
S205G
-
119.6% of wild type activity (blank has 0.5% activity)
A68D
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accumulates AcPIM4, Ac/Ac2PIM6 reduced
del175-178
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accumulates AcPIM4, no higher PIMs
del202-203
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accumulates AcPIM4, no higher PIMs
del29-44
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accumulates AcPIM4, no higher PIMs
del66-97
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accumulates AcPIM4, no higher PIMs
DELTA59-70
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mutation of the beta3-beta2 loop: mutant enzyme is still able to bind GDP with affinities in the submicromolar but PimA is completely inactivated and the ability of the protein to bind phospholipid aggregates is drastically impaired
E274A
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mutation results in complete enzyme inactivation
G164S/N167T
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Myco721.1 complemented with pimE-expressiong plasmid
G253R
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accumulates AcPIM4, no Ac/Ac2PIM6
G272V
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accumulates AcPIM4, Ac/Ac2PIM6 reduced
H118A
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mutation results in complete enzyme inactivation
Myco481
-
transposon insertion near the 3' end of the gene
Myco721
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two point mutations G163S and N166T, unstable phenotype
R201A
-
mutation results in complete enzyme inactivation
R77S/K78S/K80S/K81S
-
mutation of the four basic residues on alpha-helix 2: mutant enzyme is still able to bind GDP with affinities in the submicromolar but PimA is completely inactivated and the ability of the protein to bind phospholipid aggregates is drastically impaired
T126W
-
ability to produce phosphatidylinositol monomannoside (PIM1) is retained, enzymatic activity is similar to wild-type
T84A
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accumulates AcPIM4, Ac/Ac2PIM6 reduced
T92A
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accumulates AcPIM4, Ac/Ac2PIM6 reduced
T9A
-
mutation results in complete enzyme inactivation
W328C
-
accumulates AcPIM4, Ac/Ac2PIM6 reduced
additional information
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