Information on EC 2.4.1.54 - undecaprenyl-phosphate mannosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.54
-
RECOMMENDED NAME
GeneOntology No.
undecaprenyl-phosphate mannosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-mannose + undecaprenyl phosphate = GDP + D-mannosyl-1-phosphoundecaprenol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:undecaprenyl-phosphate D-mannosyltransferase
Requires phosphatidylglycerol.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-62-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
12-15 days after anthesis
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDPmannose + citronellyl phosphate
GDP + mannosyl-phosphorylcitronellol
show the reaction diagram
-
-
beta-isomer as product
?
GDPmannose + dolichyl phosphate
GDP + mannosyl-phosphoryldolichol
show the reaction diagram
-
-
beta-isomer as product
?
GDPmannose + ficaprenyl phosphate
GDP + mannosyl-1-phosphorylficaprenol
show the reaction diagram
GDPmannose + phospholipid
GDP + mannosyl-1-phospholipid
show the reaction diagram
-
polyprenol type, involved in glycophospholipid biosynthesis
-
-
-
GDPmannose + phytanol phosphate
GDP + D-mannosyl-1-phosphophytanol
show the reaction diagram
-
reaction is reversed by GDP, but not by GMP, enzyme is relatively specific for GDPmannose, phytanol phosphate is a poorer glycosyl acceptor than the unidentified endogenous lipid phosphate substrate
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-
r
GDPmannose + phytol phosphate
GDP + D-mannosyl-1-phosphophytol
show the reaction diagram
-
poor substrate
-
-
r
GDPmannose + polyisoprenyl phosphate
GDP + mannosyl-phosphorylpolyisoprenol
show the reaction diagram
GDPmannose + undecaprenyl phosphate
GDP + D-mannosyl-1-phosphoundecaprenol
show the reaction diagram
UDPglucose + ficaprenyl phosphate
GDP + glucosyl-1-phosphorylficaprenol
show the reaction diagram
-
in the reverse reaction UMP is no substrate
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-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDPmannose + phospholipid
GDP + mannosyl-1-phospholipid
show the reaction diagram
-
polyprenol type, involved in glycophospholipid biosynthesis
-
-
-
GDPmannose + undecaprenyl phosphate
GDP + D-mannosyl-1-phosphoundecaprenol
show the reaction diagram
-
-
Man-P-Undec is an activated mannosyl donor in micrococcal mannan biosynthesis
?
additional information
?
-
-
involved in biosynthesis of mannan
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation, can replace Mg2+ to some extent
Mn2+
-
activation, can replace Mg2+ to some extent
additional information
-
not activated by Co2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphatidylglycerol
-
inhibits in excess amount
Triton X-100
-
at high concentrations
undecaprenyl phosphate
-
inhibits in excess amount
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cutscum
-
activates
-
Detergents
-
activate
Nonidet P-40
-
activates
phosphatidylethanolamine
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activates
phosphatidylglycerol
-
requirement
Phytanol phosphate
-
stimulates
sodium lauryl sulfate
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activates
Triton X-100
-
activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02 - 0.04
ficaprenyl phosphate
-
-
0.00067
GDPmannose
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 7.5
-
0.06 M Tris-maleate buffer
7.5
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Tris-maleate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 9
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Tris buffer, about half-maximal activity at pH 6.2 and 63% of maximal activity at pH 9.0
6.2 - 8.6
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about half-maximal activity at pH 6.2 and 8.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
additional information
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 30700, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
above, rapid inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 1 year, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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useful for enzymatic synthesis of a wide variety of mannosylphosphopolyisoprenyl products
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