Information on EC 2.4.1.53 - poly(ribitol-phosphate) beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Bacillus subtilis

EC NUMBER
COMMENTARY hide
2.4.1.53
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RECOMMENDED NAME
GeneOntology No.
poly(ribitol-phosphate) beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + poly(ribitol phosphate) = UDP + (beta-D-glucosyl)poly(ribitol phosphate)
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis)
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:poly(ribitol-phosphate) beta-D-glucosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37277-61-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
W-23
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Manually annotated by BRENDA team
W-23
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose + poly(ribitol phosphate)
UDP + glucosyl-poly(ribitol phosphate)
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose + poly(ribitol phosphate)
UDP + glucosyl-poly(ribitol phosphate)
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
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absolute requirement for the addition of a divalent cation, optimal concentration: 0.01 M
MgCl2
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absolute requirement for the addition of a divalent cation, optimal concentration: 0.05 M
MnCl2
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absolute requirement for the addition of a divalent cation, optimal concentration 0.01 M
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.08
poly(ribitol phosphate)
0.12
UDP-D-glucose
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
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62% of maximal activity at pH 6.0, 70% of maximal activity at pH 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, crude preparation, stable for several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
W-23, partial
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