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EC Tree
IUBMB Comments Transfers alpha-D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide [cf. EC 2.4.1.56 (lipopolysaccharide N-acetylglucosaminyltransferase), EC 2.4.1.58 (lipopolysaccharide glucosyltransferase I) and EC 2.4.1.73 (lipopolysaccharide glucosyltransferase II)].
Word Map
2.4.1.44
angiogenic
kinase-1
vasculogenesis
vegf-induced
fms-like
neuropilin-1
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
galactosyltransferase, lipopolysaccharide alpha, 3-, galactosyltransferase, uridine diphosphogalactose-lipopolysaccharide alpha,3-, UDP-galactose:lipopolysaccharide alpha,3-galactosyltransferase, UDP-galactose:polysaccharide galactosyltransferase, uridine diphosphate galactose:lipopolysaccharide alpha-3-galactosyltransferase, uridine diphosphogalactose-lipopolysaccharide alpha,3-galactosyltransferase, Wbby,
more
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galactosyltransferase, lipopolysaccharide alpha, 3-
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galactosyltransferase, uridine diphosphogalactose-lipopolysaccharide alpha,3-
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UDP-galactose:lipopolysaccharide alpha,3-galactosyltransferase
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UDP-galactose:polysaccharide galactosyltransferase
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uridine diphosphate galactose:lipopolysaccharide alpha-3-galactosyltransferase
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uridine diphosphogalactose-lipopolysaccharide alpha,3-galactosyltransferase
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UDP-alpha-D-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose]
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hexosyl group transfer
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UDP-galactose:lipopolysaccharide 3-alpha-D-galactosyltransferase
Transfers alpha-D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide [cf. EC 2.4.1.56 (lipopolysaccharide N-acetylglucosaminyltransferase), EC 2.4.1.58 (lipopolysaccharide glucosyltransferase I) and EC 2.4.1.73 (lipopolysaccharide glucosyltransferase II)].
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UDP-alpha-D-galactose + lipopolysaccharide
UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose]
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?
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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reaction in vivo is very sensitive to subtle changes in the fatty acid structure of the membrane phospholipid
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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highly specific for UDPgalactose
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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highly specific for UDPgalactose
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
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ir
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UDP-alpha-D-galactose + lipopolysaccharide
UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose]
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?
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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reaction in vivo is very sensitive to subtle changes in the fatty acid structure of the membrane phospholipid
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism
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ir
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
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transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
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ir
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CaCl2
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no activity in absence of divalent cations, 20% of the activation with MgCl2
MgCl2
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no activity in absence of divalent cations, highest activity with MgCl2
MnCl2
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no activity in absence of divalent cations, 20% of the activation with MgCl2
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Nonsubstrate lipopolysaccharide
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competitive, Salmonella typhimurium SL1032, TV119, LT2 lipopolysaccharides; effect on several strains, overview
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Phospholipid
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required for activity
Phospholipid
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effect on several strains, overview
Phospholipid
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phosphatidylethanolamine most effective
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0.00048
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supernatant fraction
0.00284
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purified enzyme
additional information
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several bacterial strains, overview
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unsaturated fatty acid auxotrophs
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brenda
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brenda
G-30A
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brenda
G-30A
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brenda
LT2
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brenda
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bound
brenda
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bound
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brenda
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physiological function
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WbbY possesses two glycosyltransferase catalytic sites solely responsible for O1 antigen polymerization and forming a complex with the O2a glycosyltransferase WbbM. The C-terminal WbbY domain is a UDP-galactopyranoside-dependent GT-A galactosyltransferase adding beta-(1-3)-linked D-galalctopyranoside, whereas the WbbY N-terminus includes a GT-B enzyme adding alpha-(1-3)-linked D-galactofuranoside. These activities build the O1 antigen on a terminal galactopyranoside in the O2a domain
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additional information
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unidentified lipid-soluble component which contains no phosphorus is bound to the enzyme, it causes aggregation of the enzyme, but no role of the lipid in enzyme reaction has yet been established
additional information
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unidentified lipid-soluble component which contains no phosphorus is bound to the enzyme, it causes aggregation of the enzyme, but no role of the lipid in enzyme reaction has yet been established
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8.5
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4°C, several days, inactivation
489211
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one cycle of freezing and thawing causes marked loss of activity
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4°C, pH 6.8, 0.02-0.04 mg/ml protein, stable for at least 1 month
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expression in Escherichia coli
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Endo, A.; Rothfield, L.
Studies of a phospholipid-requiring bacterial enzyme. I. Purification and properties of uridine diphosphate galactose: lipopolysaccharide alpha-3-galactosyl transferase
Biochemistry
8
3500-3507
1969
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium G-30A
brenda
Muller, E.; Hinckley, A.; Rothfield, L.
Studies of phospholipid-requiring bacterial enzymes. III. Purification and properties of uridine diphosphate glucose:lipopolysaccharide glucosyltransferase I
J. Biol. Chem.
247
2614-2622
1972
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Beacham, I.R.; Silbert, D.F.
Studies on the uridine diphosphate-galactose: lipopolysaccharide galactosyltransferase reaction using a fatty acid mutant of Escherichia coli
J. Biol. Chem.
248
5310-5318
1973
Escherichia coli
brenda
Kelly, S.D.; Clarke, B.R.; Ovchinnikova, O.G.; Sweeney, R.P.; Williamson, M.L.; Lowary, T.L.; Whitfield, C.
Klebsiella pneumoniae O1 and O2ac antigens provide prototypes for an unusual strategy for polysaccharide antigen diversification
J. Biol. Chem.
294
10863-10876
2019
Klebsiella pneumoniae
brenda
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