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ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
CDP-alpha-D-glucose + D-glucose 6-phosphate
CDP + alpha,alpha-trehalose 6-phosphate
CDP-glucose + glucose 6-phosphate
CDP + alpha,alpha-trehalose 6-phosphate
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-
-
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
GDP-glucose + glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
TDP-alpha-D-glucose + D-glucose 6-phosphate
TDP + alpha,alpha-trehalose 6-phosphate
TDP-glucose + glucose 6-phosphate
TDP + alpha,alpha-trehalose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
additional information
?
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-
the trehalose-6-phosphate synthase/phosphatase (OtsA-OtsB) pathway plays an important role in the synthesis of trehalose in response to stress
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?
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
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-
-
-
?
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
ADP-alpha-D-glucose is the best substrate
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-
?
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
-
-
-
-
?
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
ADP-alpha-D-glucose is the best substrate
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-
?
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
-
in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract
-
-
?
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
-
-
-
-
?
CDP-alpha-D-glucose + D-glucose 6-phosphate
CDP + alpha,alpha-trehalose 6-phosphate
-
-
-
?
CDP-alpha-D-glucose + D-glucose 6-phosphate
CDP + alpha,alpha-trehalose 6-phosphate
-
-
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
second best substrate
-
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
second best substrate
-
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
-
in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract
-
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
GDP-glucose is the preferred glycosyl-donor
-
-
?
GDP-glucose + glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
-
-
-
-
?
GDP-glucose + glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
-
responsible for synthesis of trehalose phosphate
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate
TDP + alpha,alpha-trehalose 6-phosphate
TDP-alpha-D-glucose is the weakest substrate
-
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate
TDP + alpha,alpha-trehalose 6-phosphate
TDP-alpha-D-glucose is the weakest substrate
-
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate
TDP + alpha,alpha-trehalose 6-phosphate
-
in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
-
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
-
the enzyme exhibits less activity with UDP-alpha-D-glucose compared to ADP-alpha-D-glucose
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
-
the enzyme exhibits less activity with UDP-alpha-D-glucose compared to ADP-alpha-D-glucose
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
-
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
-
in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract
-
-
?
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Pan, Y.T.; Carroll, J.D.; Elbein, A.D.
Trehalose-phosphate synthase of Mycobacterium tuberculosis. Cloning, expression and properties of the recombinant enzyme
Eur. J. Biochem.
269
6091-6100
2002
Mycobacterium tuberculosis
brenda
Cardoso, F.S.; Castro, R.F.; Borges, N.; Santos, H.
Biochemical and genetic characterization of the pathways for trehalose metabolism in Propionibacterium freudenreichii, and their role in stress response
Microbiology
153
270-280
2007
Propionibacterium freudenreichii
brenda
Nobre, A.; Alarico, S.; Fernandes, C.; Empadinhas, N.; da Costa, M.S.
A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT
J. Bacteriol.
190
7939-7946
2008
Rubrobacter xylanophilus (B8R7Q2)
brenda
Ferreira, J.; Thevelein, J.; Hohmann, S.; Paschoalin, V.; Trugo, L.; Panek, A.
Trehalose accumulation in mutants of Saccharomyces cerevisiae deleted in the UDPG-dependent trehalose synthase-phosphatase complex
Biochim. Biophys. Acta
1335
40-50
1997
Saccharomyces cerevisiae
brenda
Asencion Diez, M.; Demonte, A.; Syson, K.; Arias, D.; Gorelik, A.; Guerrero, S.; Bornemann, S.; Iglesias, A.
Allosteric regulation of the partitioning of glucose-1-phosphate between glycogen and trehalose biosynthesis in Mycobacterium tuberculosis
Biochim. Biophys. Acta
1850
13-21
2015
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Pan, Y.; Carroll, J.; Elbein, A.
Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme
Eur. J. Biochem.
269
6091-6100
2002
Mycobacterium tuberculosis (P9WN11), Mycobacterium tuberculosis H37Rv (P9WN11)
brenda
Korte, J.; Alber, M.; Trujillo, C.; Syson, K.; Koliwer-Brandl, H.; Deenen, R.; Koehrer, K.; DeJesus, M.; Hartman, T.; Jacobs, W.J.; Bornemann, S.; Ioerger, T.; Ehrt, S.; Kalscheuer, R.
Trehalose-6-phosphate-mediated toxicity determines essentiality of OtsB2 in Mycobacterium tuberculosis in vitro and in mice
PLoS Pathog.
12
e1006043
2016
Mycobacterium tuberculosis (P9WN11), Mycobacterium tuberculosis ATCC 25618 (P9WN11)
brenda