Information on EC 2.4.1.341 - alpha-1,2-colitosyltransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.4.1.341
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RECOMMENDED NAME
GeneOntology No.
alpha-1,2-colitosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine = GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-colitose:beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine L-colitosyltransferase (configuration-inverting)
The enzyme, characterized from the bacterium Escherichia coli O55:H7, participates in the biosynthesis of an O-antigen. The reaction involves anomeric inversion, and does not require any metal ions. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine
GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
show the reaction diagram
GDP-L-fucose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine
GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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the enzyme does not require a metal ion
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine
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pH 8.0, 37°C
0.76
GDP-beta-L-colitose
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pH 8.0, 37°C
1.3
GDP-L-fucose
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pH 8.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.087
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine
Escherichia coli
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pH 8.0, 37°C
0.147
GDP-beta-L-colitose
Escherichia coli
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pH 8.0, 37°C
0.2
GDP-L-fucose
Escherichia coli
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pH 8.0, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.062
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine
Escherichia coli
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pH 8.0, 37°C
102540
0.193
GDP-beta-L-colitose
Escherichia coli
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pH 8.0, 37°C
28974
0.15
GDP-L-fucose
Escherichia coli
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pH 8.0, 37°C
282
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.2
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about 60% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
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x * 30000, N-terminal Histagged, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the gene cloned into pQE80 L for expression
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