Information on EC 2.4.1.339 - beta-1,2-mannobiose phosphorylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.339
-
RECOMMENDED NAME
GeneOntology No.
beta-1,2-mannobiose phosphorylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate = D-mannopyranose + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-mannopyranosyl-(1->2)-D-mannopyranose:phosphate alpha-D-mannosyltransferase
The enzyme, originally characterized from the thermophilic anaerobic bacterium Thermoanaerobacter sp. X514, catalyses a reversible reaction. cf. EC 2.4.1.340, 1,2-beta-oligomannan phosphorylase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannose 1-phosphate
show the reaction diagram
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannose 1-phosphate
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
show the reaction diagram
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
D-mannopyranose + alpha-D-mannose 1-phosphate
show the reaction diagram
D-fructose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
D-mannopyranose + alpha-D-mannose 1-phosphate
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1
alpha-D-mannose 1-phosphate
30°C, pH 6
4.1
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose
30°C, pH 7
0.44 - 5.1
beta-D-mannopyranosyl-(1->2)-D-mannopyranose
1.5
D-fructose
30°C, pH 6
2.1
D-mannopyranose
30°C, pH 6
0.13 - 0.19
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
33
alpha-D-mannose 1-phosphate
Listeria innocua
Q92DF6
30°C, pH 6
12
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose
Listeria innocua
Q92DF6
30°C, pH 7
12 - 35
beta-D-mannopyranosyl-(1->2)-D-mannopyranose
3.9
D-fructose
Listeria innocua
Q92DF6
30°C, pH 6
31
D-mannopyranose
Listeria innocua
Q92DF6
30°C, pH 6
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11
alpha-D-mannose 1-phosphate
Listeria innocua
Q92DF6
30°C, pH 6
638
3
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose
Listeria innocua
Q92DF6
30°C, pH 7
212413
2.3 - 79
beta-D-mannopyranosyl-(1->2)-D-mannopyranose
210726
2.7
D-fructose
Listeria innocua
Q92DF6
30°C, pH 6
117
15
D-mannopyranose
Listeria innocua
Q92DF6
30°C, pH 6
8355
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
synthetic reaction
7
phosphorolytic reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7.5
pH 4.5: about 60% of maximal activity, pH 7.5: about 60% of maximal activity, synthetic reaction
5.5 - 8
pH 5.5: about 75% of maximal activity, pH 8.0: about 60% of maximal activity, phosphorolytic reaction
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
88000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method at 20°C, crystals complexed with beta-1,2-Man2 and beta-1,2-Man3 are prepared by soaking in the cryoprotectant solution supplemented with 50 mM beta-1,2-Man2 or 10 mM beta-1,2-Man3. beta-1,2-Mannotriose is bound in a U-shape, interacting with a phosphate analog at both ends. Lin0857 has a unique dimer structure connected by a loop, and a significant open–close loop displacement iss observed for substrate entry. A long loop, which is exclusively present in Lin0857, covers the active site to limit the pocket size. A structural basis for substrate recognition and phosphorolysis is provided
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
4°C, 24 h, stable
738245
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
stable up to 50°C during 15 min of incubation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a C-terminally (His)6-tagged protein