Information on EC 2.4.1.333 - 1,2-beta-oligoglucan phosphorylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Listeria innocua

EC NUMBER
COMMENTARY hide
2.4.1.333
-
RECOMMENDED NAME
GeneOntology No.
1,2-beta-oligoglucan phosphorylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->2)-beta-D-glucosyl]n + phosphate = [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
1,2-beta-D-glucan:phosphate alpha-D-glucosyltransferase
The enzyme has been isolated from the bacterium Listeria innocua. It catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 D-glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
Listeria innocua CLIP 11262
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate
show the reaction diagram
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate
show the reaction diagram
-
-
-
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
sophorose + alpha-D-glucopyranose 1-phosphate
show the reaction diagram
-
-
-
ir
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
sophorose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
ir
sophorose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
-
1.2 - 6.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
-
1 - 6
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
-
1.2
alpha-D-glucose 1-phosphate
pH 7.5, 30C, cosubstrate sophorose
8.5
sophorose
pH 7.5, 30C, cosubstrate alpha-D-glucopyranose 1-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
31
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
Listeria innocua
Q92AT0
pH 7.5, 30C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
-
30 - 90
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
-
19 - 110
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
-
43
alpha-D-glucose 1-phosphate
Listeria innocua
Q92AT0
pH 7.5, 30C, cosubstrate sophorose
97
sophorose
Listeria innocua
Q92AT0
pH 7.5, 30C, cosubstrate alpha-D-glucopyranose 1-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
Listeria innocua
Q92AT0
pH 7.5, 30C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
194825
13 - 24
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
194824
18 - 19
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
194823
34
alpha-D-glucose 1-phosphate
Listeria innocua
Q92AT0
pH 7.5, 30C, cosubstrate sophorose
107
11
sophorose
Listeria innocua
Q92AT0
pH 7.5, 30C, cosubstrate alpha-D-glucopyranose 1-phosphate
2420
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
122900
calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21