Information on EC 2.4.1.333 - 1,2-beta-oligoglucan phosphorylase

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The expected taxonomic range for this enzyme is: Listeria innocua

EC NUMBER
COMMENTARY hide
2.4.1.333
-
RECOMMENDED NAME
GeneOntology No.
1,2-beta-oligoglucan phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->2)-beta-D-glucosyl]n + phosphate = [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
1,2-beta-D-glucan:phosphate alpha-D-glucosyltransferase
The enzyme has been isolated from the bacterium Listeria innocua. It catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 D-glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate
show the reaction diagram
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate
show the reaction diagram
-
-
-
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
sophorose + alpha-D-glucopyranose 1-phosphate
show the reaction diagram
-
-
-
ir
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
sophorose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
ir
sophorose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
1.2 - 6.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
1 - 6
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
1.2
alpha-D-glucose 1-phosphate
pH 7.5, 30°C, cosubstrate sophorose
8.5
sophorose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
31
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
Listeria innocua
Q92AT0
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
30 - 90
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
19 - 110
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
43
alpha-D-glucose 1-phosphate
Listeria innocua
Q92AT0
pH 7.5, 30°C, cosubstrate sophorose
97
sophorose
Listeria innocua
Q92AT0
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
Listeria innocua
Q92AT0
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
194825
13 - 24
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
194824
18 - 19
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
194823
34
alpha-D-glucose 1-phosphate
Listeria innocua
Q92AT0
pH 7.5, 30°C, cosubstrate sophorose
107
11
sophorose
Listeria innocua
Q92AT0
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
2420
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
1 * 120000, SDS-PAGE
122900
calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21