Information on EC 2.4.1.331 - 8-demethyltetracenomycin C L-rhamnosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Streptomyces olivaceus

EC NUMBER
COMMENTARY hide
2.4.1.331
-
RECOMMENDED NAME
GeneOntology No.
8-demethyltetracenomycin C L-rhamnosyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-beta-L-rhamnose + 8-demethyltetracenomycin C = dTDP + 8-demethyl-8-alpha-L-rhamnosyltetracenomycin C
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
elloramycin biosynthesis
-
-
Biosynthesis of type II polyketide products
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
dTDP-beta-L-rhamnose:8-demethyltetracenomycin C 3-alpha-L-rhamnosyltransferase
Isolated from Streptomyces olivaceus Tu2353. Involved in elloramycin biosynthesis. In vitro it can also utilize other 6-deoxy D- or L-hexoses.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-rhamnose + 8-demethyltetracenomycin C
dTDP + 8-demethyl-8-alpha-L-rhamnosyltetracenomycin C
show the reaction diagram
NDP-4-dehydro-L-olivose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-(4-dehydro-L-olivosyl)tetracenomycin C
show the reaction diagram
NDP-D-amicetose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-D-amicetyltetracenomycin C
show the reaction diagram
NDP-D-glucose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-D-glucosyltetracenomycin C
show the reaction diagram
NDP-D-olivose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-D-olivosyltetracenomycin C
show the reaction diagram
NDP-L-amicetose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-L-amicetyltetracenomycin C
show the reaction diagram
NDP-L-boivinose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-D-boivinosyltetracenomycin C
show the reaction diagram
NDP-L-digitose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-L-digitosyltetracenomycin C
show the reaction diagram
NDP-L-digitoxose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-L-digitoxyltetracenomycin C
show the reaction diagram
NDP-L-olivose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-L-olivosylyltetracenomycin C
show the reaction diagram
NDP-L-rhamnose + 8-demethyltetracenomycin C
NDP + 8-demethyl-8-alpha-L-rhamnosylyltetracenomycin C
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-rhamnose + 8-demethyltetracenomycin C
dTDP + 8-demethyl-8-alpha-L-rhamnosyltetracenomycin C
show the reaction diagram
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Streptomyces lividans
-
expression in Streptomyces lividans TK 24
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L309A
-
mutation in residue of the alpha/beta/alpha motif, results in 4fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
L309H
-
mutation in residue of the alpha/beta/alpha motif, results in decreases in the efficiency of deoxysugar transfer to 8-demethyltetracenomycin C
L309I
-
mutation in residue of the alpha/beta/alpha motif, results in 2fold increase in efficiency of L-rhamnose transfer to 8-demethyltetracenomycin C
L309M
-
mutation in residue of the alpha/beta/alpha motif, results in decreases in the efficiency of deoxysugar transfer to 8-demethyltetracenomycin C
L309Q
-
mutation in residue of the alpha/beta/alpha motif, results in decreases in the efficiency of deoxysugar transfer to 8-demethyltetracenomycin C
L309V
-
mutation in residue of the alpha/beta/alpha motif, results in 6fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
L309Y
-
mutation in residue of the alpha/beta/alpha motif, results in 2fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
N312Q
-
mutation in residue of the alpha/beta/alpha motif, results in 2fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
N312S
-
mutation in residue of the alpha/beta/alpha motif, results in 2fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
N312T
-
mutation in residue of the alpha/beta/alpha motif, results in 3fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
L309A
-
mutation in residue of the alpha/beta/alpha motif, results in 4fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
-
L309Q
-
mutation in residue of the alpha/beta/alpha motif, results in decreases in the efficiency of deoxysugar transfer to 8-demethyltetracenomycin C
-
L309V
-
mutation in residue of the alpha/beta/alpha motif, results in 6fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
-
L309Y
-
mutation in residue of the alpha/beta/alpha motif, results in 2fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
-
N312Q
-
mutation in residue of the alpha/beta/alpha motif, results in 2fold increase in efficiency of L-olivose transfer to 8-demethyltetracenomycin C
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
synthesis