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Information on EC 2.4.1.330 - beta-D-glucosyl crocetin beta-1,6-glucosyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Gardenia jasminoides
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beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
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UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin
UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin
(3)
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin
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UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin
(1)
UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin
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UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
(2)
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
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crocetin esters biosynthesis
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UDP-alpha-D-glucose:beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
The enzyme, characterized from the plant Gardenia jasminoides, adds a glucose to several crocetin glycosyl esters, but not to crocetin (cf. EC 2.4.1.271, crocetin glucosyltransferase).
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UDP-glucose:crocetin glucosyl ester glucosyltransferase
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SwissProt
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metabolism
the enzyme is involved in biosynthesis of crocin
physiological function
glycosyl transferase UGT94E5 is involved in synhtesis of apocarotenoid glycosyl ester crocin accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical
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UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
additional information
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UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
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UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
the enzyme is involved in biosynthesis of crocin
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UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
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UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
the enzyme is involved in biosynthesis of crocin
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UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
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UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
the enzyme is involved in biosynthesis of crocin
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additional information
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no activity with crocetin
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additional information
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no activity with crocetin
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additional information
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enzyme does not show activity against various flavonoid glucosides and coumarin glucosides as well as unnatural glucosides such as curcumin glucosides and 4-nitrophenyl glucoside
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additional information
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enzyme does not show activity against various flavonoid glucosides and coumarin glucosides as well as unnatural glucosides such as curcumin glucosides and 4-nitrophenyl glucoside
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UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
F8WKW8
the enzyme is involved in biosynthesis of crocin
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UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
F8WKW8
the enzyme is involved in biosynthesis of crocin
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?
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
F8WKW8
the enzyme is involved in biosynthesis of crocin
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beta-D-glucosyl crocetin
above 0.2 mM, substrate inhibition
additional information
no substrate inhibition with bis(beta-D-glucosyl) crocetin
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additional information
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no substrate inhibition with bis(beta-D-glucosyl) crocetin
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0.072
beta-D-glucosyl crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
0.023
bis(beta-D-glucosyl) crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
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0.036
beta-D-glucosyl crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
0.071
bis(beta-D-glucosyl) crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
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0.51
beta-D-glucosyl crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
3.1
bis(beta-D-glucosyl) crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
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cultured cells obtained from seedlings
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additional information
mRNA expression is etected in all organs tested
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additional information
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mRNA expression is etected in all organs tested
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49800
calculated from sequence
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UGT9_GARJA
444
49798
Swiss-Prot
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Nagatoshi, M.; Terasaka, K.; Owaki, M.; Sota, M.; Inukai, T.; Nagatsu, A.; Mizukami, H.
UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides
FEBS Lett.
586
1055-1061
2012
Gardenia jasminoides (F8WKW8), Gardenia jasminoides
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