Information on EC 2.4.1.330 - beta-D-glucosyl crocetin beta-1,6-glucosyltransferase

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The expected taxonomic range for this enzyme is: Gardenia jasminoides

EC NUMBER
COMMENTARY hide
2.4.1.330
-
RECOMMENDED NAME
GeneOntology No.
beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin
show the reaction diagram
UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin
show the reaction diagram
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
crocetin esters biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
The enzyme, characterized from the plant Gardenia jasminoides, adds a glucose to several crocetin glycosyl esters, but not to crocetin (cf. EC 2.4.1.271, crocetin glucosyltransferase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in biosynthesis of crocin
physiological function
glycosyl transferase UGT94E5 is involved in synhtesis of apocarotenoid glycosyl ester crocin accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
show the reaction diagram
UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
show the reaction diagram
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
show the reaction diagram
F8WKW8
the enzyme is involved in biosynthesis of crocin
-
-
?
UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
show the reaction diagram
F8WKW8
the enzyme is involved in biosynthesis of crocin
-
-
?
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
F8WKW8
the enzyme is involved in biosynthesis of crocin
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-D-glucosyl crocetin
above 0.2 mM, substrate inhibition
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.072
beta-D-glucosyl crocetin
pH 7.5, 30C; pH not specified in the publication, temperature not specified in the publication
0.023
bis(beta-D-glucosyl) crocetin
pH 7.5, 30C; pH not specified in the publication, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
beta-D-glucosyl crocetin
Gardenia jasminoides
F8WKW8
pH 7.5, 30C; pH not specified in the publication, temperature not specified in the publication
0.071
bis(beta-D-glucosyl) crocetin
Gardenia jasminoides
F8WKW8
pH 7.5, 30C; pH not specified in the publication, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.51
beta-D-glucosyl crocetin
Gardenia jasminoides
F8WKW8
pH 7.5, 30C; pH not specified in the publication, temperature not specified in the publication
28127
3.1
bis(beta-D-glucosyl) crocetin
Gardenia jasminoides
F8WKW8
pH 7.5, 30C; pH not specified in the publication, temperature not specified in the publication
84242
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
cultured cells obtained from seedlings
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49800
calculated from sequence