Information on EC 2.4.1.330 - beta-D-glucosyl crocetin beta-1,6-glucosyltransferase

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The expected taxonomic range for this enzyme is: Gardenia jasminoides

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EC NUMBER
COMMENTARY hide
2.4.1.330
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RECOMMENDED NAME
GeneOntology No.
beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin
show the reaction diagram
UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin
show the reaction diagram
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
crocetin esters biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
The enzyme, characterized from the plant Gardenia jasminoides, adds a glucose to several crocetin glycosyl esters, but not to crocetin (cf. EC 2.4.1.271, crocetin glucosyltransferase).
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in biosynthesis of crocin
physiological function
glycosyl transferase UGT94E5 is involved in synhtesis of apocarotenoid glycosyl ester crocin accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
show the reaction diagram
UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
show the reaction diagram
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
UDP + crocin
show the reaction diagram
F8WKW8
the enzyme is involved in biosynthesis of crocin
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-
?
UDP-alpha-D-glucose + beta-D-glucosyl crocetin
UDP + beta-D-gentiobiosyl crocetin
show the reaction diagram
F8WKW8
the enzyme is involved in biosynthesis of crocin
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-
?
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
F8WKW8
the enzyme is involved in biosynthesis of crocin
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-
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-D-glucosyl crocetin
above 0.2 mM, substrate inhibition
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.072
beta-D-glucosyl crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
0.023
bis(beta-D-glucosyl) crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
beta-D-glucosyl crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
0.071
bis(beta-D-glucosyl) crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.51
beta-D-glucosyl crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
3.1
bis(beta-D-glucosyl) crocetin
pH 7.5, 30°C; pH not specified in the publication, temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
cultured cells obtained from seedlings
Manually annotated by BRENDA team
additional information
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49800
calculated from sequence
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.330)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)