Information on EC 2.4.1.33 - mannuronan synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.33
-
RECOMMENDED NAME
GeneOntology No.
mannuronan synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-alpha-D-mannuronate + [(1->4)-beta-D-mannuronosyl]n = GDP + [(1->4)-beta-D-mannuronosyl]n+1
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alginate biosynthesis I (algal)
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alginate biosynthesis II (bacterial)
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Fructose and mannose metabolism
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alginate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
GDP-alpha-D-mannuronate:mannuronan D-mannuronatetransferase
The enzyme catalyses the polymerization of beta-D-mannuronate residues into a mannuronan polymer, an intermediate in the biosynthesis of alginate. It is found in brown algae and in alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
CAS REGISTRY NUMBER
COMMENTARY hide
37257-31-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
marine brown alga
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-glucuronate 1-phosphate + (alginate)n
phosphate + ?
show the reaction diagram
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25% of activity with GDP-D-mannuronate
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-
?
alpha-D-mannuronate 1-phosphate + (alginate)n
phosphate + (alginate)n+1
show the reaction diagram
-
25% of activity with GDP-D-mannuronate
-
-
?
GDP-D-mannuronate + (alginate)n
GDP + (alginate)n+1
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-D-mannuronate + (alginate)n
GDP + (alginate)n+1
show the reaction diagram
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-
-
-
ir
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MnCl2
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no essential requirement, at 0.25 mM: 15% increase of activity, inhibition above 0.75 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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5% inhibition
MnCl2
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at 0.25 mM: 15% increase of activity, above 0.75 mM: 80% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-GMP
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slight stimulation
GDP
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0.3 mM: 15% stimulation
additional information
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GTP, UTP, CTP, TTP, a mixture of NAD+ and NADP+ have no significant effect
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 8.5
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65% of maximal activity at pH 5.6 and pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
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15 min, 23% remaining activity
36
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15 min, 72% remaining activity
45
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15 min, 60% remaining activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 3 days, 80% remaining activity
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0-4°C, unstable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE