Information on EC 2.4.1.329 - sucrose 6F-phosphate phosphorylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Thermoanaerobacterium thermosaccharolyticum

EC NUMBER
COMMENTARY hide
2.4.1.329
-
RECOMMENDED NAME
GeneOntology No.
sucrose 6F-phosphate phosphorylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sucrose 6F-phosphate + phosphate = alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
sucrose 6F-phosphate:phosphate 1-alpha-D-glucosyltransferase
The enzyme, isolated from the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum, catalyses the reversible phosphorolysis of sucrose 6F-phosphate. It also acts on sucrose with lower activity.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose
sucrose 6F-phosphate + phosphate
show the reaction diagram
-
-
-
?
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
sucrose 6F-phosphate + phosphate
show the reaction diagram
sucrose + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose
show the reaction diagram
51% compared to the activity with sucrose 6F-phosphate
-
-
r
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sucrose 6F-phosphate + phosphate
alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
show the reaction diagram
D9TT09
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.6
alpha-D-glucopyranose 1-phosphate
pH 6.0, 55C; pH 6.0, 55C
41.6
beta-D-fructofuranose
pH 6.0, 55C
15.1
beta-D-fructofuranose 6-phosphate
pH 6.0, 55C; pH 6.0, 55C
6.9
phosphate
pH 6.5, 55C; pH 6.5, 55C
12.7
sucrose 6F-phosphate
pH 6.5, 55C; pH 6.5, 55C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14.4
alpha-D-glucopyranose 1-phosphate
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.0, 55C; pH 6.0, 55C
16.9
beta-D-fructofuranose
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.0, 55C
24.2
beta-D-fructofuranose 6-phosphate
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.0, 55C; pH 6.0, 55C
59.3
phosphate
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.5, 55C; pH 6.5, 55C
66.2
Sucrose
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.5, 55C
82.6
sucrose 6F-phosphate
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.5, 55C; pH 6.5, 55C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
alpha-D-glucopyranose 1-phosphate
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.0, 55C; pH 6.0, 55C
194794
0.4
beta-D-fructofuranose
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.0, 55C
2782
0.4 - 1.6
beta-D-fructofuranose 6-phosphate
194795
8.6
phosphate
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.5, 55C; pH 6.5, 55C
16
0.9
Sucrose
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.5, 55C
55
6.5
sucrose 6F-phosphate
Thermoanaerobacterium thermosaccharolyticum
D9TT09
pH 6.5, 55C; pH 6.5, 55C
30906
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
synthesis reaction; synthetic reaction
6.5
phosphorolysis; phosphorolytic reaction
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
x * 56000 SDS-PAGE, x * 56700, calculated
56700
x * 56000 SDS-PAGE, x * 56700, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
half-life 60 h; half-life: 60 h
79
melting temperature; melting temperature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H344Y
decreased ratio of activity on phosphorylated fructose over fructose
K301A
displays similar activities as the wild type enzyme
R134A
decreased ratio of activity on phosphorylated fructose over fructose
R135A
displays similar activities as the wild type enzyme