Information on EC 2.4.1.327 - aclacinomycin-T 2-deoxy-L-fucose transferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Streptomyces galilaeus

EC NUMBER
COMMENTARY hide
2.4.1.327
-
RECOMMENDED NAME
GeneOntology No.
aclacinomycin-T 2-deoxy-L-fucose transferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T = dTDP + aclacinomycin S
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
aclacinomycin biosynthesis
-
-
Biosynthesis of type II polyketide products
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
dTDP-2-deoxy-beta-L-fucose:7-(alpha-L-rhodosaminyl)aklavinone 2-deoxy-alpha-L-fucosyltransferase
The enzyme, isolated from the bacterium Streptomyces galilaeus, is involved in the biosynthesis of other aclacinomycins. Also acts on idarubicin. It will slowly add a second 2-deoxy-L-fucose unit to aclacinomycin S in vitro.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
show the reaction diagram
dTDP-L-2-deoxyfucose + aclacinomycin S
dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S
show the reaction diagram
dTDP-L-2-deoxyfucose + aclacinomycin T
dTDP + aclacinomycin S
show the reaction diagram
idarubicin + dTDP-L-2-deoxyfucose
dTDP + 4'-O-(alpha-L-fucosyl)idarubicin
show the reaction diagram
rhodosaminyl aklavinone + dTDP-L-daunosamine
dTDP + daunosaminyl-rhodosaminyl-aklavinone
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1489 - 0.531
dTDP-L-2-deoxyfucose
0.94
dTDP-L-daunosamine
-
in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25C
0.138
Idarubicin
-
in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25C
0.1044 - 0.1091
rhodosaminyl aklavinone
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09
Idarubicin
Streptomyces galilaeus
-
in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25C
0.035 - 1.09
rhodosaminyl aklavinone
-
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA resin column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-