Information on EC 2.4.1.326 - aklavinone 7-L-rhodosaminyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces galilaeus

EC NUMBER
COMMENTARY hide
2.4.1.326
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RECOMMENDED NAME
GeneOntology No.
aklavinone 7-L-rhodosaminyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-beta-L-rhodosamine + aklavinone = dTDP + aclacinomycin T
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
aclacinomycin biosynthesis
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Biosynthesis of antibiotics
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Biosynthesis of type II polyketide products
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aclacinomycin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dTDP-beta-L-rhodosamine:aklavinone 7-alpha-L-rhodosaminyltransferase (configuration-inverting)
Isolated from the bacterium Streptomyces galilaeus. Forms a complex with its accessory protein AknT, and has very low activity in its absence. The enzyme can also use dTDP-2-deoxy-beta-L-fucose. Involved in the biosynthesis of other aclacinomycins.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-4-amino-2-deoxyrhamnose + aklavinone
dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnopyranosyl)aklavinone
show the reaction diagram
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-
-
-
?
dTDP-4-amino-2-deoxyrhamnose + aklavinone
dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnosyl)aklavinone
show the reaction diagram
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-
-
-
?
dTDP-beta-L-rhodosamine + aklavinone
dTDP + aclacinomycin T
show the reaction diagram
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-
-
-
?
dTDP-beta-L-rhodosamine + aklavinone
dTDP + rhodosaminyl-aklavinone
show the reaction diagram
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-
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?
dTDP-L-2-deoxyfucose + aklavinone
dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone
show the reaction diagram
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-
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?
dTDP-L-2-deoxyfucose + epsilon-rhodomycinone
dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)-epsilon-rhodomycinone
show the reaction diagram
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-
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?
dTDP-L-daunosamine + aklavinone
dTDP + ?
show the reaction diagram
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-
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-rhodosamine + aklavinone
dTDP + aclacinomycin T
show the reaction diagram
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-
-
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?
dTDP-beta-L-rhodosamine + aklavinone
dTDP + rhodosaminyl-aklavinone
show the reaction diagram
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?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AKnT protein
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0031 - 0.013
aklavinone
0.4072
dTDP-4-amino-2-deoxyrhamnose
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in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
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0.28 - 0.349
dTDP-beta-L-rhodosamine
0.156 - 0.2878
dTDP-L-2-deoxyfucose
0.046
epsilon-rhodomycinone
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with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 0.004
aklavinone
0.003
dTDP-4-amino-2-deoxyrhamnose
Streptomyces galilaeus
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in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
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0.0083 - 0.16
dTDP-beta-L-rhodosamine
0.001 - 0.004
dTDP-L-2-deoxyfucose
0.001
epsilon-rhodomycinone
Streptomyces galilaeus
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with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity column chromatography, and gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli XL1 Blue MRF' cells
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