Information on EC 2.4.1.324 - 7-deoxyloganetin glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.324
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RECOMMENDED NAME
GeneOntology No.
7-deoxyloganetin glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 7-deoxyloganetin = UDP + 7-deoxyloganin
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:7-deoxyloganetin O-D-glucosyltransferase
Isolated from the plants Catharanthus roseus (Madagascar periwinkle) and Gardenia jasminoides (cape jasmine). With Gardenia it also acts on genipin. Involved in loganin and secologanin biosynthesis. Does not react with 7-deoxyloganetate. cf. EC 2.4.1.323 7-deoxyloganetic acid glucosyltransferase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 7-deoxyloganetin
UDP + 7-deoxyloganin
show the reaction diagram
UDP-alpha-D-glucose + genipin
UDP + ?
show the reaction diagram
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-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 7-deoxyloganetin
UDP + 7-deoxyloganin
show the reaction diagram
F8WKW5
the enzyme is involved in loganin and secologanin biosynthesis
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.202 - 0.61
7-deoxyloganetin
8.82
genipin
pH 8.0, 30°C
0.117 - 0.17
UDP-alpha-D-glucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0355 - 0.13
7-deoxyloganetin
1.04
genipin
Gardenia jasminoides
F8WKW5
pH 8.0, 30°C
0.032 - 0.16
UDP-alpha-D-glucose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.179 - 0.21
7-deoxyloganetin
29160
0.12
genipin
Gardenia jasminoides
F8WKW5
pH 8.0, 30°C
37648
0.291 - 1
UDP-alpha-D-glucose
364
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the mRNA level gradually increases during the early stage of fruit ripening, whereas it remains low in the mature fruits
Manually annotated by BRENDA team
preferential expression
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
x * 55000, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55000, calculated
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
molecular modeling and ligand docking studies. The enzyme shows maximum binding affinity towards 7-deoxyloganetin. Apigenin, kaempferol and naringenin show very poor binding affinity towards the protein’s acceptor site
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as N-terminal fusion proteins with a His6 tag in Escherichia coli strain JM109
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is strongly induced by phytohormone treatments: 15fold increase in the expression after 24 h of methyl jasmonate application, 10fold after 12 h of treatment with salicylic acid
mRNA level is markedly increased by adding methyl jasmonate to the cell cultures and the pattern of up-regulation correlated well with the pattern of increased enzymatic activity; the mRNA level gradually increases during the early stage of fruit ripening