Information on EC 2.4.1.323 - 7-deoxyloganetic acid glucosyltransferase

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The expected taxonomic range for this enzyme is: Catharanthus roseus

EC NUMBER
COMMENTARY hide
2.4.1.323
-
RECOMMENDED NAME
GeneOntology No.
7-deoxyloganetic acid glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 7-deoxyloganetate = UDP + 7-deoxyloganate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
secologanin and strictosidine biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:7-deoxyloganetate O-D-glucosyltransferase
Isolated from the plant Catharanthus roseus (Madagascar periwinkle). Involved in loganin and secologanin biosynthesis. Does not react with 7-deoxyloganetin. cf. EC 2.4.1.324 7-deoxyloganetin glucosyltransferase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is involved in loganin and secologanin biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 7-deoxyloganetate
UDP + 7-deoxyloganate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 7-deoxyloganetate
UDP + 7-deoxyloganate
show the reaction diagram
U3U992
the enzyme is involved in loganin and secologanin biosynthesis
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.38
7-deoxyloganetate
pH 8.0, 30°C
0.088
UDP-alpha-D-glucose
pH 8.0, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.325
7-deoxyloganetate
Catharanthus roseus
U3U992
pH 8.0, 30°C
0.13
UDP-alpha-D-glucose
Catharanthus roseus
U3U992
pH 8.0, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.064
7-deoxyloganetate
Catharanthus roseus
U3U992
pH 8.0, 30°C
29159
1.5
UDP-alpha-D-glucose
Catharanthus roseus
U3U992
pH 8.0, 30°C
364
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
preferentially expressed in internal phloem associated parenchyma cells
Manually annotated by BRENDA team
preferentially expressed in internal phloem associated parenchyma cells
Manually annotated by BRENDA team
preferentially expressed in internal phloem associated parenchyma cells
Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme, purified by nickel nitrilotriacetic acid affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli