Information on EC 2.4.1.322 - devancosaminyl-vancomycin vancosaminetransferase

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The expected taxonomic range for this enzyme is: Amycolatopsis orientalis

EC NUMBER
COMMENTARY hide
2.4.1.322
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RECOMMENDED NAME
GeneOntology No.
devancosaminyl-vancomycin vancosaminetransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-beta-L-vancosamine + desvancosaminyl-vancomycin = dTDP + vancomycin
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of vancomycin group antibiotics
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SYSTEMATIC NAME
IUBMB Comments
dTDP-beta-L-vancomycin:desvancosaminyl-vancomycin beta-L-vancosaminetransferase
The enzyme, isolated from the bacterium Amycolatopsis orientalis, catalyses the ultimate step in the biosynthesis of the antibiotic vancomycin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-acosamine + devancosaminyl-vancomycin
dTDP + ?
show the reaction diagram
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?
dTDP-beta-L-daunosamine + devancosaminyl-vancomycin
dTDP + ?
show the reaction diagram
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-
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?
dTDP-beta-L-vancosamine + desvancosaminyl-vancomycin
dTDP + vancomycin
show the reaction diagram
dTDP-beta-L-vancosamine + devancosaminyl-vancomycin
dTDP + vancomycin
show the reaction diagram
UDP-4-epi-L-vancosamine + devancosaminyl-vancomycin
UDP + epi-L-vancomycin
show the reaction diagram
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epivancomycin is a hybrid teicoplanin/vancomycin glycopeptide
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?
UDP-4-epi-vancosamine + 4-deoxy-glucosyl-teicoplanin aglycone
UDP + 4-epi-vancosaminyl-(1,2)-4-deoxy-coplanin heptapeptide
show the reaction diagram
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-
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?
UDP-4-epi-vancosamine + devancosaminyl-vancomycin
UDP + epivancomycin
show the reaction diagram
UDP-beta-L-vancosamine + desvancosaminyl-vancomycin
dUDP + vancomycin
show the reaction diagram
UDP-L-epi-vancosamine + devancosaminyl-vancomycin
UDP + ?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-vancosamine + devancosaminyl-vancomycin
dTDP + vancomycin
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016 - 0.027
desvancosaminyl-vancomycin
0.232
dTDP-beta-L-acosamine
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pH not specified in the publication, temperature not specified in the publication
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0.038
dTDP-beta-L-daunosamine
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pH not specified in the publication, temperature not specified in the publication
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2
UDP-L-epi-vancosamine
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pH 7.0, 37C, wild-type enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 1.12
desvancosaminyl-vancomycin
2.25
dTDP-beta-L-acosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
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2.13
dTDP-beta-L-daunosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
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0.002 - 1.12
UDP-L-epi-vancosamine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.7
dTDP-beta-L-acosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
194822
56.7
dTDP-beta-L-daunosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
194821
0.0033 - 0.56
UDP-L-epi-vancosamine
29158
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, X-ray crystal structure of GtfD as a complex with TDP and desvancosaminyl-vancomycin at 2.0 A resolution
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D13A
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5630fold decrease in kcat for UDP-L-epi-vancosamine
T10A
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172fold decrease in kcat/Km for UDP-L-epi-vancosamine
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis