Information on EC 2.4.1.322 - devancosaminyl-vancomycin vancosaminetransferase

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The expected taxonomic range for this enzyme is: Amycolatopsis orientalis

EC NUMBER
COMMENTARY hide
2.4.1.322
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RECOMMENDED NAME
GeneOntology No.
devancosaminyl-vancomycin vancosaminetransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-beta-L-vancosamine + desvancosaminyl-vancomycin = dTDP + vancomycin
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of vancomycin group antibiotics
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SYSTEMATIC NAME
IUBMB Comments
dTDP-beta-L-vancomycin:desvancosaminyl-vancomycin beta-L-vancosaminetransferase
The enzyme, isolated from the bacterium Amycolatopsis orientalis, catalyses the ultimate step in the biosynthesis of the antibiotic vancomycin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-acosamine + devancosaminyl-vancomycin
dTDP + ?
show the reaction diagram
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-
-
-
?
dTDP-beta-L-daunosamine + devancosaminyl-vancomycin
dTDP + ?
show the reaction diagram
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-
-
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?
dTDP-beta-L-vancosamine + desvancosaminyl-vancomycin
dTDP + vancomycin
show the reaction diagram
dTDP-beta-L-vancosamine + devancosaminyl-vancomycin
dTDP + vancomycin
show the reaction diagram
UDP-4-epi-L-vancosamine + devancosaminyl-vancomycin
UDP + epi-L-vancomycin
show the reaction diagram
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epivancomycin is a hybrid teicoplanin/vancomycin glycopeptide
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?
UDP-4-epi-vancosamine + 4-deoxy-glucosyl-teicoplanin aglycone
UDP + 4-epi-vancosaminyl-(1,2)-4-deoxy-coplanin heptapeptide
show the reaction diagram
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-
-
-
?
UDP-4-epi-vancosamine + devancosaminyl-vancomycin
UDP + epivancomycin
show the reaction diagram
UDP-beta-L-vancosamine + desvancosaminyl-vancomycin
dUDP + vancomycin
show the reaction diagram
UDP-L-epi-vancosamine + devancosaminyl-vancomycin
UDP + ?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-vancosamine + devancosaminyl-vancomycin
dTDP + vancomycin
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016 - 0.027
desvancosaminyl-vancomycin
0.232
dTDP-beta-L-acosamine
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pH not specified in the publication, temperature not specified in the publication
0.038
dTDP-beta-L-daunosamine
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pH not specified in the publication, temperature not specified in the publication
2
UDP-L-epi-vancosamine
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pH 7.0, 37°C, wild-type enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 1.12
desvancosaminyl-vancomycin
2.25
dTDP-beta-L-acosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
2.13
dTDP-beta-L-daunosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
0.002 - 1.12
UDP-L-epi-vancosamine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.7
dTDP-beta-L-acosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
194822
56.7
dTDP-beta-L-daunosamine
Amycolatopsis orientalis
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pH not specified in the publication, temperature not specified in the publication
194821
0.0033 - 0.56
UDP-L-epi-vancosamine
29158
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42881
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x * 42881, calculation from sequence
44000
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x * 44000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, X-ray crystal structure of GtfD as a complex with TDP and desvancosaminyl-vancomycin at 2.0 A resolution
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D13A
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5630fold decrease in kcat for UDP-L-epi-vancosamine
T10A
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172fold decrease in kcat/Km for UDP-L-epi-vancosamine
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis