Information on EC 2.4.1.320 - 1,4-beta-mannosyl-N-acetylglucosamine phosphorylase

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The expected taxonomic range for this enzyme is: Bacteroides thetaiotaomicron

EC NUMBER
COMMENTARY hide
2.4.1.320
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RECOMMENDED NAME
GeneOntology No.
1,4-beta-mannosyl-N-acetylglucosamine phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation
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SYSTEMATIC NAME
IUBMB Comments
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine:phosphate alpha-D-mannosyltransferase
The enzyme isolated from the anaerobic bacterium Bacteroides thetaiotaomicron is involved in the degradation of host-derived N-glycans.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
show the reaction diagram
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
2.7
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
pH 5.5, 30°C
2.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C; pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
27
N,N'-diacetylchitobiose
pH 5.5, 30°C, synthetic reaction
3.8
N-acetyl-D-glucosamine
pH 5.5, 30°C; pH 5.5, 30°C, synthetic reaction
0.6
phosphate
pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
240
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
22
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C
25
alpha-D-mannose 1-phosphate
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C; pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
22
N,N'-diacetylchitobiose
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, synthetic reaction
37
N-acetyl-D-glucosamine
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C; pH 5.5, 30°C, synthetic reaction
240
phosphate
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
110
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
19807
9.6
alpha-D-mannose 1-phosphate
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction
638
1.9
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction
174854
0.8
N,N'-diacetylchitobiose
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, synthetic reaction
1354
9.6
N-acetyl-D-glucosamine
Bacteroides thetaiotaomicron
Q8A8X4, Q8A8Y4
pH 5.5, 30°C, synthetic reaction
300
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
pH 5.5, 30°C, substrate: N,N'-diacetylchitobiose
258
pH 5.5, 30°C, substrate: N-acetyl-D-glucosamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
; phosphorolytic reaction; synthetic reaction
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
4 * 35000, SDS-PAGE; 4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
37823
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence; 4 * 37823, calculated from sequence
147000
gel filtration; gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10.5
4°C, 24 h stable; 4°C, 24 h, stable
725534
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
15 min stable up to; 15 min, stable up to
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha; expression in Escherichia coli