Information on EC 2.4.1.319 - beta-1,4-mannooligosaccharide phosphorylase

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The expected taxonomic range for this enzyme is: Ruminococcus albus

EC NUMBER
COMMENTARY hide
2.4.1.319
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RECOMMENDED NAME
GeneOntology No.
beta-1,4-mannooligosaccharide phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->4)-beta-D-mannosyl]n + phosphate = [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mannan degradation
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SYSTEMATIC NAME
IUBMB Comments
1,4-beta-D-mannooligosaccharide:phosphate alpha-D-mannosyltransferase
The enzyme, isolated from the ruminal bacterium Ruminococcus albus, catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannosyl-D-glucose + phosphate
D-glucose + alpha-D-mannosyl 1-phosphate
show the reaction diagram
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
show the reaction diagram
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-
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r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
show the reaction diagram
-
-
-
r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
show the reaction diagram
best substrate for the synthetic reaction
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-
r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
show the reaction diagram
-
-
-
r
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
show the reaction diagram
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-
-
r
beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
show the reaction diagram
-
-
-
r
beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
D-mannose + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
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r
[(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
[(1->4)-beta-D-mannosyl]3 + phosphate
show the reaction diagram
[(1->4)-beta-D-mannosyl]2 + phosphate
D-mannose + alpha-D-mannose 1-phosphate
show the reaction diagram
[(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
[(1->4)-beta-D-mannosyl]4 + phosphate
show the reaction diagram
-
-
-
-
?
[(1->4)-beta-D-mannosyl]3 + phosphate
[(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
-
?
[(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate
[(1->4)-beta-D-mannosyl]5 + phosphate
show the reaction diagram
-
-
-
-
?
[(1->4)-beta-D-mannosyl]4 + phosphate
[(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
-
?
[(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate
[(1->4)-beta-D-mannosyl]6 + phosphate
show the reaction diagram
-
-
-
-
?
[(1->4)-beta-D-mannosyl]5 + phosphate
[(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
-
?
[(1->4)-beta-D-mannosyl]6 + alpha-D-mannose 1-phosphate
[(1->4)-beta-D-mannosyl]7 + phosphate
show the reaction diagram
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-
-
-
?
[(1->4)-beta-D-mannosyl]6 + phosphate
[(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
-
?
[(1->4)-beta-D-mannosyl]n + phosphate
[(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
show the reaction diagram
[(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
[(1->4)-beta-D-mannosyl]n + phosphate
show the reaction diagram
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the enzyme catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers. It acts on D-glucose derivatives at the C2- and C3-positions, including deoxy- and deoxyfluoro-analogues and epimers, but not on those substituted at the C6-position. It shows high synthetic activity toward the following oligosaccharides: beta-linked glucobioses, maltose, N,N'-diacetylchitobiose, and beta-1,4-mannooligosaccharides. Particularly, beta-1,4-mannooligosaccharides serve as significantly better acceptor substrates for the enzyme than D-glucose. In the phosphorolytic reactions, RaMP2 has weak activity toward beta-1,4-mannobiose but efficiently degrades beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose. It is thought to catalyze the phosphorolysis of beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose to produce alpha-D-mannose 1-phosphate and beta-1,4-mannobiose
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r
additional information
?
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The enzyme also catalyzes the reaction of EC 2.4.1.281, 4-O-beta-D-mannosyl-D-glucose phosphorylase
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[(1->4)-beta-D-mannosyl]n + phosphate
[(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
28.4
4-O-beta-D-mannosyl-D-glucose
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pH 6.5, 37C, cosubstrate: phosphate, phosphorolysis reaction
4.48
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
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pH 6.5, 37C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
4.55 - 5.29
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
3.21 - 7.59
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
7.94 - 13.3
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
30.7 - 44.5
beta-D-mannopyranosyl-(1->4)-D-mannopyranose
44.5
[(1->4)-beta-D-mannosyl]2
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pH 6.5, 37C, cosubstrate: phosphate, phosphorolysis reaction
7.94 - 13.3
[(1->4)-beta-D-mannosyl]3
3.21 - 7.59
[(1->4)-beta-D-mannosyl]4
4.55 - 5.29
[(1->4)-beta-D-mannosyl]5
4.48
[(1->4)-beta-D-mannosyl]6
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pH 6.5, 37C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.7
4-O-beta-D-mannosyl-D-glucose
Ruminococcus albus
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pH 6.5, 37C, cosubstrate: phosphate, phosphorolysis reaction
16
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
Ruminococcus albus
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pH 6.5, 37C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
20.5 - 31.9
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
31.2 - 33.1
beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
27.5 - 34.6
beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man
7.06 - 45.1
beta-D-mannopyranosyl-(1->4)-D-mannopyranose
45.1
[(1->4)-beta-D-mannosyl]2
Ruminococcus albus
-
pH 6.5, 37C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction
27.5 - 34.6
[(1->4)-beta-D-mannosyl]3
31.2 - 33.1
[(1->4)-beta-D-mannosyl]4
20.5 - 31.9
[(1->4)-beta-D-mannosyl]5
16
[(1->4)-beta-D-mannosyl]6
Ruminococcus albus
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pH 6.5, 37C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.729
4-O-beta-D-mannosyl-D-glucose
Ruminococcus albus
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pH 6.5, 37C, cosubstrate: phosphate, phosphorolysis reaction
42698
0.159 - 1.47
[(1->4)-beta-D-mannosyl]2
12262
2.6 - 3.46
[(1->4)-beta-D-mannosyl]3
10433
4.11 - 10.3
[(1->4)-beta-D-mannosyl]4
10434
3.87 - 7.01
[(1->4)-beta-D-mannosyl]5
10435
3.57
[(1->4)-beta-D-mannosyl]6
Ruminococcus albus
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pH 6.5, 37C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction
19806
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.03
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pH 6.5, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7)
Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
209000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in presence and absence of beta-(1->4)-mannobiose, and comparison with 4-O-beta-D-mannosyl-D-glucose phosphorylase MP1. The structures of the two enzymes differ at the +1 subsite of the substrate-binding pocket. Three loops are proposed to determine the different substrate specificities. Loop2 restricts the derivatives at the C6 position of the +1 substrate residue in beta-(1,4)-mannooligosaccharide phosphorylase MP2
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 9.5
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21
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expression in Escherichia coli BL21 (DE3)
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