Information on EC 2.4.1.315 - diglucosyl diacylglycerol synthase (1,6-linking)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.315
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RECOMMENDED NAME
GeneOntology No.
diglucosyl diacylglycerol synthase (1,6-linking)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol = 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
type I lipoteichoic acid biosynthesis (S. aureus)
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Glycerolipid metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 6-glucosyltransferase
The enzyme is found in several bacterial species. The enzyme from Bacillus subtilis is specific for glucose [1]. The enzyme from Mycoplasma genitalium can incoporate galactose with similar efficiency, but forms mainly 1,2-diacyl-diglucopyranosyl-sn-glycerol in vivo [3]. The enzyme from Staphylococcus aureus can also form glucosyl-glycero-3-phospho-(1'-sn-glycerol) [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
ATCC 33530
Uniprot
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
UDP-alpha-D-galactose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
9% of the specific activity with 1,2-diacyl-sn-glycerol
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
UDP-alpha-D-glucose + 1,2-diacyl-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol + UDP
show the reaction diagram
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?
UDP-alpha-D-glucose + glycero-3-phospho-(1'-sn-glycerol)
alpha-D-glucopyranosyl-glycero-3-phospho-(1'-sn-glycerol) + UDP
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
show the reaction diagram
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dioleoylphosphatidylglycerol
kcat linearly increases with dioleoylphosphatidylglycerol concentration
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28C
0.27
1,2-diacyl-sn-glycerol
donor UDP-alpha-D-galactose, pH 8.0, 28C
0.234 - 0.479
UDP-alpha-D-galactose
0.05 - 0.087
UDP-alpha-D-glucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
Mycoplasma genitalium
Q9ZB73
donor UDP-alpha-D-galactose, pH 8.0, 28C
1.13
1,2-diacyl-sn-glycerol
Mycoplasma genitalium
Q9ZB73
donor UDP-alpha-D-galactose, pH 8.0, 28C
0.03 - 1.1
UDP-alpha-D-galactose
0.004 - 0.27
UDP-alpha-D-glucose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
Mycoplasma genitalium
Q9ZB73
donor UDP-alpha-D-galactose, pH 8.0, 28C
16021
4
1,2-diacyl-sn-glycerol
Mycoplasma genitalium
Q9ZB73
donor UDP-alpha-D-galactose, pH 8.0, 28C
2063
0.063 - 5
UDP-alpha-D-galactose
891
0.08 - 3.1
UDP-alpha-D-glucose
364
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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