Information on EC 2.4.1.314 - ginsenoside Rd glucosyltransferase

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The expected taxonomic range for this enzyme is: Panax

EC NUMBER
COMMENTARY hide
2.4.1.314
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RECOMMENDED NAME
GeneOntology No.
ginsenoside Rd glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + ginsenoside Rd = UDP + ginsenoside Rb1
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ginsenosides biosynthesis
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ginsenoside metabolism
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:ginsenoside-Rd beta-1,6-glucosyltransferase
The glucosyl group forms a 1->6 bond to the glucosyloxy moiety at C-20 of ginsenoside Rd. Isolated from sanchi ginseng (Panax notoginseng).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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129% activity at 5 mM
Mg2+
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111.9% activity at 5 mM
Mn2+
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133.2% activity at 5 mM
additional information
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the activity of the enzyme is not apparently affected by Na+ or K+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
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8.2% residual activity at 5 mM
Cu2+
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2.2% residual activity at 5 mM
Fe2+
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2.3% residual activity at 5 mM
Hg2+
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complete inhibition at 5 mM
Zn2+
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complete inhibition at 5 mM
additional information
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the activity of the enzyme is not apparently affected by EDTA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
ginsenoside Rd
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in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C
0.32
UDP-alpha-D-glucose
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in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0003
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crude extract, at pH 8.5 and 35°C
0.044
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after 145.8fold purification, at pH 8.5 and 35°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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more than 60% activity between pH 7.0 and 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
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x * 36000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 36000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, HiTrap Q anion-exchange chromatography, and Sephadex G-25 gel filtration
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
6 days after treatment, the enzyme activity is elicited by 0.2 mM 2-hydroxyethyl jasmonate and induced by 0.2 mM methyl jasmonate
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the activity of the enzyme is up-regulated 6 days after treatment with 0.01 mM N,N'-dicyclohexylcarbodiimide
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