Information on EC 2.4.1.313 - protein O-mannose beta-1,3-N-acetylgalactosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.313
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RECOMMENDED NAME
GeneOntology No.
protein O-mannose beta-1,3-N-acetylgalactosaminyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + 3-O-[N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein] = UDP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein]
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Mannose type O-glycan biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-galactosamine:N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl-threonyl-[protein] 3-beta-N-acetyl-D-galactosaminyltransferase
The human protein is specific for UDP-N-acetyl-alpha-D-galactosamine as donor [1]. The enzyme is involved in the formation of a phosphorylated trisaccharide on a threonine residue of alpha-dystroglycan, an extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
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beta-1,3-N-acetylgalactosaminyltransferase2 B3GALNT2 and protein O-mannose beta-1,4-Nacetylglucosaminyltransferase GTDC2 act coordinately on O-mannose to synthesize the O-mannosyl glycan GalNAc-beta-3-GlcNAc-beta-4-Man present in alpha-dystroglycan. Kinase SGK196 phosphorylates the 6-position of O-mannose using ATP
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-galactosamine + 3-O-[N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein]
UDP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein]
show the reaction diagram
UDP-N-acetyl-alpha-D-galactosamine + 4-nitrophenyl-beta-D-glucose
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show the reaction diagram
2% activity compared to GlcNAc-beta-benzyl
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UDP-N-acetyl-alpha-D-galactosamine + calf fetuin
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show the reaction diagram
the enzyme efficiently transfers GalNAc to N-glycans of fetal calf fetuin, which is treated with neuraminidase and beta-galactosidase
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UDP-N-acetyl-alpha-D-galactosamine + Gal-beta(1->3)(GlcNAcbeta(1->6))GalNAcalpha1-O-4-nitrophenyl
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show the reaction diagram
best acceptor substrate, 185% activity compared to GlcNAc-beta-benzyl
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?
UDP-N-acetyl-alpha-D-galactosamine + Glc-NAcbeta(1->6)GalNAcalpha1-O-4-nitrophenyl
?
show the reaction diagram
19% activity compared to GlcNAc-beta-benzyl
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?
UDP-N-acetyl-alpha-D-galactosamine + GlcNAc-beta-benzyl
?
show the reaction diagram
100% activity
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?
UDP-N-acetyl-alpha-D-galactosamine + GlcNAcbeta(1->3)GalNAcalpha-4-nitrophenyl
UDP + GalNAcbeta(1->3)GlcNAcbeta(1->3)GalNAcalpha-4-nitrophenyl
show the reaction diagram
8% activity compared to GlcNAc-beta-benzyl
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?
UDP-N-acetyl-alpha-D-galactosamine + GlcNAcbeta(1->4)GlcNAcbeta1-O-benzyl
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show the reaction diagram
29% activity compared to GlcNAc-beta-benzyl
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additional information
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the enzyme shows no activity toward any glycolipid, Gal-beta-4-mitrophenyl, Gal-beta-O-4-nitrophenyl, Gal-NAc-alpha-benzyl, GalNAc-beta-4-nitrophenyl, Glc-alpha-4-nitrophenyl, GlcA-beta-4-nitrophenyl, Fuc-alpha-4-nitrophenyl, Xyl-alpha-4-nitrophenyl, Xyl-beta-4-nitrophenyl, Man-alpha-4-nitrophenyl, lactoside-benzyl, lactosylceramide, galactocenebroside type 1, paragloboside, globoside, Galbeta(1->4)GalNAc-alpha-4-nitrophenyl, Galbeta(1->3)GlcNAc-beta-4-nitrophenyl, and Galbeta(1->3)GalNAcalpha(core1)-4-nitrophenyl
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-galactosamine + 3-O-[N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein]
UDP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein]
show the reaction diagram
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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highest expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
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x * 55000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 55000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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the enzyme is N-glycosylated on both Asn-116 and Asn-174. This modification is necessary for its secretion in breast cancer cells
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in HEK-293T cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is exclusively upregulated in the majority of breast cancers
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine