Information on EC 2.4.1.311 - chloroorienticin B synthase

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The expected taxonomic range for this enzyme is: Amycolatopsis orientalis

EC NUMBER
COMMENTARY hide
2.4.1.311
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RECOMMENDED NAME
GeneOntology No.
chloroorienticin B synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin = dTDP + chloroorienticin B
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of vancomycin group antibiotics
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SYSTEMATIC NAME
IUBMB Comments
dTDP-L-4-epi-vancosamine:desvancosaminyl-vancomycin vancosaminyltransferase
The enzyme from the bacterium Amycolatopsis orientalis is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-4-epi-vancosamine + aglucovancomycin
dTDP + chloroorienticin C
show the reaction diagram
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin
dTDP + chloroorienticin B
show the reaction diagram
dTDP-beta-L-4-epi-vancosamine + epivancomycin
dTDP + chloroeremomycin
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin
dTDP + chloroorienticin B
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.107
devancoaminyl vancomycin
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pH 7.5, 37°C
0.218
dTDP-beta-L-4-epi-vancosamine
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pH 7.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038
devancoaminyl vancomycin
Amycolatopsis orientalis
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pH 7.5, 37°C
0.038
dTDP-beta-L-4-epi-vancosamine
Amycolatopsis orientalis
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pH 7.5, 37°C
additional information
additional information
Amycolatopsis orientalis
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GtfA transfers 4-epi-vancosamine to vancomycin aglycone or epivancomycin at much reduced rates of less than 0.0008/s
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2.8 A crystal structures of GtfA complexes with vancomycin and the natural monoglycosylated peptide substrate devancoaminyl vancomycin
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
heterologously expressed as a C-terminal His6-tagged protein in Escherichia coli
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