Information on EC 2.4.1.310 - vancomycin aglycone glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Amycolatopsis orientalis

EC NUMBER
COMMENTARY hide
2.4.1.310
-
RECOMMENDED NAME
GeneOntology No.
vancomycin aglycone glucosyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + vancomycin aglycone = UDP + devancosaminyl-vancomycin
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of vancomycin group antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:vancomycin aglycone 48-O-beta-D-glucosyltransferase
The enzyme from the bacterium Amycolatopsis orientalis is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + teicoplanin aglycone
UDP + ?
show the reaction diagram
UDP-alpha-D-glucose + vancomycin aglycone
UDP + devancoaminyl vancomycin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + vancomycin aglycone
UDP + devancoaminyl vancomycin
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
-
vancomycin aglycone
-
severe substrate inhibition above 0.6 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45 - 3.3
UDP-alpha-D-glucose
0.25
vancomycin aglycone
-
pH 9, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0022 - 0.72
UDP-alpha-D-glucose
0.283
vancomycin aglycone
Amycolatopsis orientalis
-
pH 9, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00067 - 4.6
UDP-alpha-D-glucose
364
0.88
vancomycin aglycone
Amycolatopsis orientalis
-
pH 9, 37°C
19703
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
x * 44000, C-terminal His6-tagged version of GtfB, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, 1.8 resolution
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli as a hexahistidine-tagged protein
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D13A
-
kcat/Km is 9.2% compared to the wild-type value
D332A
-
kcat/Km is 0.16% compared to the wild-type value
H125A
-
kcat/Km is 3.8fold higher than the wild-type value
D13A
-
kcat/Km is 9.2% compared to the wild-type value
-
D332A
-
kcat/Km is 0.16% compared to the wild-type value
-
H125A
-
kcat/Km is 3.8fold higher than the wild-type value
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis