Information on EC 2.4.1.31 - laminaribiose phosphorylase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.31
-
RECOMMENDED NAME
GeneOntology No.
laminaribiose phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
laminaribiose degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
3-beta-D-glucosyl-D-glucose:phosphate alpha-D-glucosyltransferase
Also acts on 1,3-beta-D-oligoglucans. Differs in specificity from EC 2.4.1.30 (1,3-beta-oligoglucan phosphorylase) and EC 2.4.1.97 (1,3-beta-D-glucan phosphorylase).
CAS REGISTRY NUMBER
COMMENTARY hide
37257-29-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Astasia ocellata
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
3-O-beta-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
?
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-arabino-hexopyranose
?
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + arbutin
?
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + cellobiose
phosphate + oligosaccharides
show the reaction diagram
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
show the reaction diagram
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucuronic acid
3-O-beta-D-glucopyranosyl-D-glucuronic acid + phosphate
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
3-O-beta-D-glucopyranosyl-D-mannose + phosphate
show the reaction diagram
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
?
show the reaction diagram
the major product (83%) in the reaction with D-xylose is 3-O-beta-D-glucopyranosyl-D-xylose and the other fraction (17%) contains 2-O-beta-D-glucopyranosyl-D-xylose as the major component
-
-
r
alpha-D-glucose 1-phosphate + laminaribiose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + laminaribiosyl-beta-1,4-glucose
?
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritetraose
?
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritriose
?
show the reaction diagram
alpha-D-glucose 1-phosphate + m-nitrophenyl-beta-D-glucoside
?
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
?
show the reaction diagram
alpha-D-glucose 1-phosphate + p-nitrophenyl-beta-D-glucoside
?
show the reaction diagram
-
-
-
-
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
?
show the reaction diagram
alpha-D-glucose 1-phosphate + salicin
?
show the reaction diagram
-
-
-
-
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
show the reaction diagram
laminariheptaose + phosphate
?
show the reaction diagram
laminarihexaose + phosphate
?
show the reaction diagram
laminaripentaose + phosphate
?
show the reaction diagram
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
show the reaction diagram
laminaritetraose + phosphate
?
show the reaction diagram
only weak phosphorylation
-
-
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
show the reaction diagram
laminaritriose + phosphate
?
show the reaction diagram
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
Astasia ocellata
-
slight activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-deoxyglucose
-
25-30% inhibition at 20 mM
D-fructose
-
25-30% inhibition at 20 mM
D-glucosamine
-
25-30% inhibition at 20 mM
D-glucose
D-mannose
-
25-30% inhibition at 20 mM
diphosphate
-
10% inhibition at 1 mM, pH 7
fructose 6-phosphate
-
10% inhibition at 1 mM
HgCl2
-
10% inhibition at 1 mM
imidazole
-
weak, 25% inhibition at 0.4 M
laminaribiose
NADP+
-
10% inhibition at 1 mM
p-hydroxymercuribenzoate
-
40% inhibition at 0.2 mM and 80% inhibition at 2 mM
phloridzin
-
10% inhibition at 1 mM
additional information
-
not inhibited by 1 mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD+, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, glyceric acid 3-phosphate, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
Astasia ocellata
-
markedly increased activity
additional information
-
not activated by 1mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, 3-phosphate-glyceric acid, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7
2-deoxy-D-glucose
pH 6.0, 40°C, reverse reaction
0.4 - 19
D-glucose
2.1
D-glucose 1-phosphate
-
with glucose addition
0.48 - 5
laminaribiose
6
laminaritriose
-
with phosphate addition
0.14 - 2.5
phosphate
additional information
additional information
-
linear within certain limits
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
2-deoxy-D-glucose
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
1.4
D-glucose
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
6.5 - 19
laminaribiose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
1,5-Anhydro-D-glucitol
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
2926
1.4
2-deoxy-D-glucose
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
531
3.5
D-glucose
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
35
0.024
D-glucuronic acid
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
1498
0.009
D-mannose
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
216
0.064
D-xylose
Acholeplasma laidlawii
A9NG65
pH 6.0, 40°C, reverse reaction
115
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.4 - 9.8
laminaribiose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24
-
D-glucose + D-glucose 1-phosphate, in DEAE-eluate
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
reverse phosphorolytic reaction using alpha-D-glucose 1-phosphate as the donor and D-glucose as the acceptor has optimum at pH 6.0
6.3 - 6.9
-
same optimum pH for for forward and reverse reaction
6.5
-
assay at
6.8
6.8-7.0 optimal pH
7.2
-
in Tris-HCl buffer
7.5
highest apparent phosphorolytic activity on laminaribiose
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
SDS-PAGE, monomer
120000
-
2 * 120000, SDS-PAGE
200000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 10.5
stable at 4°C for 24h
719230
6 - 9
-
719125
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21
Astasia ocellata
-
5%, 9%, 24% and 37% loss of activity after 2 h, 6 h, 24 h and 48 h, respectively
40
-
isoenzymes F0 and F2, stable below
45
over 30 min at pH 7
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
aqueous extracts not stable
Astasia ocellata
-
stable to repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-14°C, 3 months stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3 isoforms: F0, FI, FII
-
protamine sulfate and ammonium sulfate precipitation, calcium phosphate gel adsorption and DEAE-cellulose chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli