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3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
3-O-beta-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-arabino-hexopyranose
?
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
alpha-D-glucose 1-phosphate + arbutin
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + cellobiose
phosphate + oligosaccharides
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucuronic acid
3-O-beta-D-glucopyranosyl-D-glucuronic acid + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
3-O-beta-D-glucopyranosyl-D-mannose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
?
the major product (83%) in the reaction with D-xylose is 3-O-beta-D-glucopyranosyl-D-xylose and the other fraction (17%) contains 2-O-beta-D-glucopyranosyl-D-xylose as the major component
-
-
r
alpha-D-glucose 1-phosphate + laminaribiose
?
alpha-D-glucose 1-phosphate + laminaribiosyl-beta-1,4-glucose
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritetraose
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritriose
?
alpha-D-glucose 1-phosphate + m-nitrophenyl-beta-D-glucoside
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
?
alpha-D-glucose 1-phosphate + p-nitrophenyl-beta-D-glucoside
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
?
alpha-D-glucose 1-phosphate + salicin
?
-
-
-
-
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
laminariheptaose + phosphate
?
laminarihexaose + phosphate
?
laminaripentaose + phosphate
?
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
laminaritetraose + phosphate
?
only weak phosphorylation
-
-
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
?
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
additional information
?
-
3-beta-D-glucosyl-D-glucose + phosphate

D-glucose + alpha-D-glucose 1-phosphate
Astasia ocellata
-
the enzyme is specific for the alpha-isomer
-
r
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose

?
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + cellobiose

phosphate + oligosaccharides
Astasia ocellata
-
-
oligosaccharides with two or more 1,3-linked glucose residues attached to the nonreducing residue of cellobiose
r
alpha-D-glucose 1-phosphate + cellobiose
phosphate + oligosaccharides
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose

laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
Astasia ocellata
-
at equilibrium synthesis of laminaribiose is favoured
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
Astasia ocellata
-
specific for alpha-D-glucose 1-phosphate as glucosyl donor
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
-
-
-
-
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
-
initial rate of synthetic reaction is 4 times faster than that of phosphorolytic reaction
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
-
specific for alpha-D-glucose 1-phosphate as glucosyl donor
amount of individual products depends on substrate concentration and reaction time
-
alpha-D-glucose 1-phosphate + laminaribiose

?
Astasia ocellata
-
-
-
-
r
alpha-D-glucose 1-phosphate + laminaribiose
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritriose

?
Astasia ocellata
-
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritriose
?
-
-
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside

?
Astasia ocellata
-
-
-
-
?
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside

?
Astasia ocellata
-
-
-
-
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
?
-
-
-
-
r
laminaribiose + phosphate

alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
laminariheptaose + phosphate

?
only weak phosphorylation
-
-
r
laminariheptaose + phosphate
?
only weak phosphorylation
-
-
r
laminarihexaose + phosphate

?
only weak phosphorylation
-
-
r
laminarihexaose + phosphate
?
only weak phosphorylation
-
-
r
laminaripentaose + phosphate

?
only weak phosphorylation
-
-
r
laminaripentaose + phosphate
?
only weak phosphorylation
-
-
r
laminaripentaose + phosphate

laminaritetraose + alpha-D-glucose 1-phosphate
-
-
-
-
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritetraose + phosphate

laminaritriose + alpha-D-glucose 1-phosphate
-
-
-
-
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritriose + phosphate

?
only weak phosphorylation
-
-
r
laminaritriose + phosphate
?
only weak phosphorylation
-
-
r
laminaritriose + phosphate

laminaribiose + alpha-D-glucose 1-phosphate
-
-
-
-
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
-
-
r
additional information

?
-
Astasia ocellata
-
in presence of arsenate, laminaribiose is completely converted into glucose
-
-
-
additional information
?
-
Astasia ocellata
-
weak or no activity observed with other carbohydrates
-
-
-
additional information
?
-
-
EC 1.4.1.31 and EC 1.4.1.30 catalyse the same reaction but with different quantitative specificity, EC 1.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose while the opposite behaviour is observed with EC 1.4.1.31
-
-
-
additional information
?
-
-
arsenolysis of laminaribiose is observed, but not that of glucose 1-phosphate
-
-
-
additional information
?
-
-
weak or no activity observed with other carbohydrates
-
-
-
additional information
?
-
-
weak or no activity observed with other carbohydrates
-
-
-
additional information
?
-
on reverse phosphorolysis enzyme is most active on glucose as acceptor and is weakly active on several monosaccharides (e.g. mannose) with alpha-D-glucose 1-phosphate as donor
-
-
-
additional information
?
-
on reverse phosphorolysis enzyme is most active on glucose as acceptor and is weakly active on several monosaccharides (e.g. mannose) with alpha-D-glucose 1-phosphate as donor
-
-
-
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2-deoxyglucose
-
25-30% inhibition at 20 mM
D-fructose
-
25-30% inhibition at 20 mM
D-glucosamine
-
25-30% inhibition at 20 mM
D-mannose
-
25-30% inhibition at 20 mM
diphosphate
-
10% inhibition at 1 mM, pH 7
fructose 6-phosphate
-
10% inhibition at 1 mM
HgCl2
-
10% inhibition at 1 mM
imidazole
-
weak, 25% inhibition at 0.4 M
NADP+
-
10% inhibition at 1 mM
p-hydroxymercuribenzoate
-
40% inhibition at 0.2 mM and 80% inhibition at 2 mM
phloridzin
-
10% inhibition at 1 mM
additional information
-
not inhibited by 1 mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD+, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, glyceric acid 3-phosphate, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
-
D-glucose

-
25-30% inhibition at 10 mM
D-glucose
-
substrate inhibition at high concentration
D-glucose
higher concentrations of glucose cause competitive substrate inhibition
laminaribiose

-
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Marechal, L.R.
beta-1,3-Oligoglucan: orthophosphate glucosyltransferases from Euglena gracilis. II. Comparative studies between laminaribiose- and beta-1,3-oligoglucan phosphorylase
Biochim. Biophys. Acta
146
431-442
1967
Euglena gracilis
brenda
Kitaoka, M.; Hayashi, K.
Carbohydrate-processing phosphorolytic enzymes
Trends Glycosci. Glycotechnol.
14
35-50
2002
Euglena gracilis
-
brenda
Manners, D.J.; Taylor, D.C.
Studies on carbohydrate metabolizing enzymes. XVI. Specificity of laminaribiose phosphorylase from Astasia ocellata
Arch. Biochem. Biophys.
121
443-451
1967
Astasia ocellata
brenda
Goldemberg, S.H.; Marechal, L.R.; De Souza, B.C.
beta-1,3-Oligoglucan: orthophosphate glucosyltransferase from Euglena gracilis
J. Biol. Chem.
241
45-50
1966
Euglena gracilis
brenda
Kitaoka, M.; Sasaki, T.; Taniguchi, H.
Purification and properties of laminaribiose phosphorylase (EC 2.4 1.31) from Euglena gracilis Z
Arch. Biochem. Biophys.
304
508-514
1993
Euglena gracilis
brenda
Kitaoka, M.; Matsuoka, Y.; Mori, K.; Nishimoto, M.; Hayashi, K.
Characterization of a bacterial laminaribiose phosphorylase
Biosci. Biotechnol. Biochem.
76
343-348
2012
Paenibacillus sp. (D7UT17), Paenibacillus sp. YM-1 (D7UT17)
brenda
Nihira, T.; Saito, Y.; Kitaoka, M.; Nishimoto, M.; Otsubo, K.; Nakai, H.
Characterization of a laminaribiose phosphorylase from Acholeplasma laidlawii PG-8A and production of 1,3-beta-D-glucosyl disaccharides
Carbohydr. Res.
361C
49-54
2012
Acholeplasma laidlawii (A9NG65)
brenda