Information on EC 2.4.1.303 - UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-galactosyltransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.4.1.303
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RECOMMENDED NAME
GeneOntology No.
UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-galactosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactose:N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-beta-galactosyltransferase
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli O7:K1 (VW187). Requires Mn2+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside
UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
show the reaction diagram
UDP-alpha-D-galactose + 1-O-(16-phenoxyhexadecyl-diphosphoryl)-alpha-D-glucopyranoside
UDP + 1-O-(16-phenoxyhexadecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
show the reaction diagram
UDP-alpha-D-galactose + 1-O-(6-phenoxyhex-1-yl-diphosphoryl)-alpha-D-glucopyranoside
UDP + 1-O-(6-phenoxyhexadecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
show the reaction diagram
synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol. The enzyme does not require a specific length of the lipid chain but can act on GlcNAc-alpha-PO3-PO3-(CH2)R-OPh substrates where R can be 6, 11 or 16 carbons
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?
UDP-alpha-D-galactose + GlcNAc-alpha-PO3-PO3-(CH2)11-O-phenyl
UDP + D-Gal-beta-(1->3)-GlcNAc-alpha-PO3-PO3-(CH2)11-O-phenyl
show the reaction diagram
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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5 mM, yields 38% activity, respectively, compared with 5 mM MnCl2
Mg2+
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5 mM, yields 11% activity, respectively, compared with 5 mM MnCl2
Mn2+
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optimal activity in presence of 2.5 mM Mn2+. Without addition of divalent metal the activity is less than 4.5% of the activity in presence of Mn2+
Zn2+
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5 mM, yields 9% activity, respectively, compared with 5 mM MnCl2
additional information
requirement for divalent metal ion
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CHAPS
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activity is diminished in the presence of CHAPS at concentrations of 0.125–0.6%
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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detergents in the assay do not increase glycosyl transfer
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08
1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside
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pH 7.0, 37°C
1.2
UDP-alpha-D-galactose
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pH 7.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
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broad optimum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as a GST-fusion protein encoded by pCM227 in DH5alpha
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine