Information on EC 2.4.1.302 - L-demethylnoviosyl transferase

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
2.4.1.302
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RECOMMENDED NAME
GeneOntology No.
L-demethylnoviosyl transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-4-O-demethyl-beta-L-noviose + novobiocic acid = dTDP + demethyldecarbamoyl novobiocin
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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novobiocin biosynthesis
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Novobiocin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dTDP-4-O-demethyl-beta-L-noviose:novobiocic acid 7-O-[4-O-demethyl-L-noviosyl]transferase
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-6-deoxy-L-glucose + novobiocic acid
dTDP + ?
show the reaction diagram
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-
-
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?
dTDP-beta-L-noviose + N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide
dTDP + N-[7-[(6-deoxy-5-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-chromen-3-yl]-4-hydroxy-3-methylbenzamide
show the reaction diagram
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-
-
-
?
dTDP-beta-L-noviose + novobiocic acid
dTDP + demethyldecarbamoyl novobiocin
show the reaction diagram
dTDP-L-4-dehydro-6-deoxy-glucose + novobiocic acid
dTDP + ?
show the reaction diagram
-
-
-
-
?
dTDP-L-noviose + 4-nitrophenol
dTDP + ?
show the reaction diagram
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kcat/Km is 208000fold lower compared to the value for the natural substrate novobiocic acid
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?
dTDP-L-noviose + 7-hydroxy-4-methylcoumarin
dTDP + ?
show the reaction diagram
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kcat/Km is 142000fold lower compared to the value for the natural substrate novobiocic acid
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-
?
dTDP-L-noviose + 7-hydroxycoumarin
dTDP + ?
show the reaction diagram
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kcat/Km is 172000fold lower compared to the value for the natural substrate novobiocic acid
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-
?
dTDP-L-noviose + cyclonovobiocic acid
dTDP + ?
show the reaction diagram
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kcat/KM is 30% compared to the value for the natural substrate novobiocic acid
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?
dTDP-xylose + novobiocic acid
dTDP + ?
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-noviose + novobiocic acid
dTDP + demethyldecarbamoyl novobiocin
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dTDP-beta-L-rhamnose
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novobiocic acid
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above 0.06 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.039
cyclonovobiocic acid
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pH 6.0, 22°C
0.0086
dTDP-beta-L-noviose
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pH 6.0, 22°C
0.0086
N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide
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pH 6.0, temperature not specified in the publication
0.032 - 0.039
novobiocic acid
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
118
cyclonovobiocic acid
Streptomyces niveus
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pH 6.0, 22°C
226
dTDP-beta-L-noviose
Streptomyces niveus
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pH 6.0, 22°C
290
N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide
Streptomyces sp.
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pH 6.0, temperature not specified in the publication
219 - 313
novobiocic acid
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.047
4-nitrophenol
Streptomyces niveus
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pH 6.0, 22°C
80
0.069
7-hydroxy-4-methylcoumarin
Streptomyces niveus
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pH 6.0, 22°C
11493
0.057
7-hydroxycoumarin
Streptomyces niveus
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pH 6.0, 22°C
4113
3026
cyclonovobiocic acid
Streptomyces niveus
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pH 6.0, 22°C
42423
3300
N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide
Streptomyces sp.
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pH 6.0, temperature not specified in the publication
42424
5615 - 9781
novobiocic acid
5875
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0835
dTDP-beta-L-rhamnose
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pH 6.0, 22°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
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assay at room temperature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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x * 40000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 40000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
C-terminal His8 fusion protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a C-terminal His8 fusion protein
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