Information on EC 2.4.1.30 - 1,3-beta-oligoglucan phosphorylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Euglena gracilis

EC NUMBER
COMMENTARY hide
2.4.1.30
-
RECOMMENDED NAME
GeneOntology No.
1,3-beta-oligoglucan phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->3)-beta-D-glucan:phosphate alpha-D-glucosyltransferase
Does not act on laminarin. Differs in specificity from EC 2.4.1.31 (laminaribiose phosphorylase) and EC 2.4.1.97 (1,3-beta-D-glucan phosphorylase).
CAS REGISTRY NUMBER
COMMENTARY hide
37257-28-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain Z
-
-
Manually annotated by BRENDA team
Ochromonas malhamensis
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
show the reaction diagram
alpha-phenylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-alpha-phenylglucoside + phosphate
show the reaction diagram
-
very low activity
-
-
r
arbutin + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylarbutin + phosphate
show the reaction diagram
-
-
-
-
r
beta-methylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-beta-methylglucoside + phosphate
show the reaction diagram
-
-
-
-
r
beta-phenylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-beta-phenylglucoside + phosphate
show the reaction diagram
-
-
-
-
r
cellobiose + alpha-D-glucose 1-phosphate
?
show the reaction diagram
-
-
-
-
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
show the reaction diagram
-
-
-
-
r
gentiobiose + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylgentiobiose + phosphate
show the reaction diagram
-
-
-
-
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
show the reaction diagram
laminaribiose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
show the reaction diagram
laminaribiosyl-beta-1,4-glucose + alpha-D-glucose 1-phosphate
laminaritriosyl-beta-1,4-glucose + phosphate
show the reaction diagram
-
-
-
-
r
laminaribiosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritriosyl-p-hydroquinone + phosphate
show the reaction diagram
-
-
-
-
r
laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritriosyl-saligenin + phosphate
show the reaction diagram
laminaridextrins + alpha-D-glucose 1-phosphate
?
show the reaction diagram
-
-
-
-
r
laminariheptaose + alpha-D-glucose 1-phosphate
laminarioctaose + phosphate
show the reaction diagram
-
-
-
-
r
laminaripentaose + alpha-D-glucose 1-phosphate
laminarihexaose + phosphate
show the reaction diagram
-
low activity
-
-
r
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
laminaritetraose + alpha-D-glucose 1-phosphate
laminaripentaose + phosphate
show the reaction diagram
-
low activity
-
-
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate
laminaripentaosyl-saligenin + phosphate
show the reaction diagram
laminaritriose + alpha-D-glucose 1-phosphate
laminaritetraose + phosphate
show the reaction diagram
-
-
-
-
r
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
r
laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritetraosyl-p-hydroquinone + phosphate
show the reaction diagram
laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritetraosyl-saligenin + phosphate
show the reaction diagram
paramylon + phosphate
?
show the reaction diagram
Ochromonas malhamensis
-
-
-
-
r
salicin + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylsalicin + phosphate
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroquinone
-
strongly inhibits in absence of 2-mercaptoethanol
iodoacetate
-
-
p-mercuribenzoate
-
strong inhibition
UMP
-
in phosphorolysis, 30% inhibition at 3 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
additional information
-
absolute requirement for sulfhydryl groups
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40
glucose
-
-
1.8
glucose 1-phosphate
-
+ laminaribiose
4
laminaribiose
-
-
2
Laminaripentaose
-
-
3
laminaritetraose
-
-
4.5
laminaritriose
-
-
2 - 3
phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3
-
laminaribiose
0.5
-
glucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
-
glucose or laminaribiose as acceptor
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
-
assay at
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
without 2-mercaptoethanol: loss of 80% activity after 2 weeks and over 95% after 8 weeks in enzyme extracts, with 2-mercaptoethanol: loss of 50% activity after 8 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Ochromonas malhamensis
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis