Information on EC 2.4.1.296 - anthocyanidin 3-O-coumaroylrutinoside 5-O-glucosyltransferase

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The expected taxonomic range for this enzyme is: Petunia x hybrida

EC NUMBER
COMMENTARY hide
2.4.1.296
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RECOMMENDED NAME
GeneOntology No.
anthocyanidin 3-O-coumaroylrutinoside 5-O-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + an anthocyanidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] = UDP + an anthocyanidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:anthocyanidin-3-O-[3-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucosyltransferase
Isolated from the plant Petunia hybrida. It does not act on an anthocyanidin 3-O-rutinoside
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
two copies gene
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside
UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside
show the reaction diagram
UDP-alpha-D-glucose + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
UDP + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
show the reaction diagram
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i.e. delphinidin 3-(4-coumaroyl)-rutinoside
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-
?
UDP-alpha-D-glucose + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
UDP + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
show the reaction diagram
-
i.e. petunidin 3-(4-coumaroyl)-rutinoside
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside
UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside
show the reaction diagram
UDP-alpha-D-glucose + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
UDP + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
show the reaction diagram
-
i.e. delphinidin 3-(4-coumaroyl)-rutinoside
-
-
?
UDP-alpha-D-glucose + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
UDP + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
show the reaction diagram
-
i.e. petunidin 3-(4-coumaroyl)-rutinoside
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no stimulation by 1 mM Mg2+ or Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no inhibition by EDTA at 5 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
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pH 8.3, 30C
0.003
petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
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pH 8.3, 30C
0.22
UDP-alpha-D-glucose
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pH 8.3, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.05
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purified native enzyme, pH 8.3, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.75
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chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
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x * 52000, SDS-PAGE
52163
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x * 52163, PH1, sequence calculation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
30fold from flowers, by ammonium sulfate fractionation, desalting gel filtration, separation of the 5-O-glucosyltransferase from the anthocyanidin 3-O-glucosyltransferase by chromatofocusing, followed by ultrafiltration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
PH1, cloning by hybridization screening, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression in Saccharomyces cerevisiae strain G1315
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