Information on EC 2.4.1.292 - GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.292
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RECOMMENDED NAME
GeneOntology No.
GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol = 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
protein N-glycosylation (bacterial)
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-galactosamine:GalNAc-alpha-(1->4)-GalNAc-alphja-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol 4-alpha-N-acetyl-D-galactosaminyltransferase
Isolated from Campylobacter jejuni. Part of a bacterial N-linked glycosylation pathway.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains FA1090 and N400, gene pglH
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Manually annotated by BRENDA team
strains FAM18 and Z2491, gene pglH
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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PglH is a glycosyltransferase protein containing the conserved EX7E sequence motif
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
show the reaction diagram
additional information
?
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
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assay at
8.5
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
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x * 41000, recombinant His-tagged PglH, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged PglH from Escherichia coli by nickel affinity chromatography
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recombinant N-terminally octahistidine-tagged enzyme from Escherichia coli strain BL-21 RIL by nickel affinity chromatography, followed by cleavage of the His8-tag by TEV protease and another step of nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene pglH, cj1129,par of the pgl gene cluster
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gene pglH, expression of the N-terminally octahistidine-tagged enzyme containing a TEV cleavage sequence in Escherichia coli strain BL-21 RIL using a modified auto-induction method
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gene pglH, overexpression of His-tagged PglH in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D170A
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site-directed mutagenesis
D306A
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site-directed mutagenesis
D307A
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site-directed mutagenesis
E171A
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site-directed mutagenesis
E179A
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site-directed mutagenesis
E265A
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site-directed mutagenesis
E273A
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site-directed mutagenesis
E308A
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site-directed mutagenesis
E316A
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site-directed mutagenesis
E346A
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site-directed mutagenesis
E354A
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site-directed mutagenesis
E41A
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site-directed mutagenesis
E49A
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site-directed mutagenesis
R191A
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site-directed mutagenesis
H371R
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site-directed mutagenesis, strain FA 1090, the mutation is sufficient to restore PglH activity in the wild-type FA1090 strain
additional information