Information on EC 2.4.1.288 - galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

Word Map on EC 2.4.1.288
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The expected taxonomic range for this enzyme is: Mycobacterium

EC NUMBER
COMMENTARY hide
2.4.1.288
-
RECOMMENDED NAME
GeneOntology No.
galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase
Isolated from Mycobacterium tuberculosis. The enzyme adds approximately twenty-eight galactofuranosyl residues with alternating 1->5 and 1->6 links forming a galactan domain with approximately thirty galactofuranosyl residues. Involved in the formation of the cell wall in mycobacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
364048-29-3
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-2''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-3''-deoxy-alpha-D-xylo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-3-deoxy-alpha-D-threo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-5''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-6''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-arabinofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-arabinofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactofuranose + 12-phenoxy-dodec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside
UDP + ?
show the reaction diagram
UDP-alpha-D-galactofuranose + 19-phenoxy-nondec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside
UDP + ?
show the reaction diagram
UDP-alpha-D-galactofuranose + 9-phenoxy-non-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside
UDP + ?
show the reaction diagram
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
show the reaction diagram
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3
beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3
UDP + ?
show the reaction diagram
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3
?
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3
UDP + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
best donor-acceptor combination, dual recognition of both the terminal C-5 and C-6 hydroxyl groups of the acceptor substrate are required for GlfT2 activity
-
-
?
UDP-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactofuranose + dec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside
UDP + ?
show the reaction diagram
-
-
-
-
-
UDP-alpha-D-galactofuranose + octyl beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside
UDP + octyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
essential for catalysis
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2R,3R,4R,5R)-2-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl hydrogen ((E)-((3R,4R,5S)-5-((R)-1,2-dihydroxyethyl)-3,4-dihydroxydihydrofuran-2(3H)-ylidene)fluoromethyl)phosphonate
-
-
(2R,3R,4R,5R)-2-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl hydrogen ((Z)-((3R,4R,5S)-5-((R)-1,2-dihydroxyethyl)-3,4-dihydroxydihydrofuran-2(3H)-ylidene)fluoromethyl)phosphonate
-
-
(3S,4S)-3-(1,2-dihydroxyethyl)-4-hydroxy-1-(2-phenylethyl)tetrahydrothiophenium chloride
-
sulfonium ion compound as analogue of the donor substrate, uridine diphosphategalactofuranose. The compound contains moieties that mimic both galactofuranose and uridine diphosphate domains, and carries a permanent positive charge to mimic the oxocarbenium ion-like transition state. About 60% inhibition at 4 mM
(3S,4S)-3-(1,2-dihydroxyethyl)-4-hydroxy-1-[2-(4-nitrophenyl)ethyl]tetrahydrothiophenium bromide
-
sulfonium ion compound as analogue of the donor substrate, uridine diphosphategalactofuranose. The compound contains moieties that mimic both galactofuranose and uridine diphosphate domains, and carries a permanent positive charge to mimic the oxocarbenium ion-like transition state. About 50% inhibition at 4 mM
UDP-3''-deoxy-alpha-D-xylo-hexofuranose
-
68% inhibition at 1.25 mM
UDP-5''-O-methyl-alpha-D-galactofuranose
-
15% inhibition at 1.25 mM
UDP-5-deoxy-alpha-D-galactofuranose
-
10% inhibition at 1.25 mM
UDP-6''-O-methyl-alpha-D-galactofuranose
-
26% inhibition at 1.25 mM
UDP-alpha-D-arabinofuranose
-
less than 1% inhibition at 1.25 mM
additional information
-
not inhibited by UDP-2''-O-methyl-alpha-D-galactofuranose
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6 - 1.78
beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3
1.9
dTDP-alpha-D-galactofuranose
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.5
UDP-3-deoxy-alpha-D-threo-hexofuranose
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.9
UDP-5-deoxy-alpha-D-galactofuranose
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
1
UDP-6-deoxy-alpha-D-galactofuranose
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.9
UDP-alpha-D-arabinofuranose
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.0038 - 1
UDP-alpha-D-galactofuranose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 7.2
beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3
1.62
dTDP-alpha-D-galactofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.02
UDP-3-deoxy-alpha-D-threo-hexofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.17
UDP-5-deoxy-alpha-D-galactofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
1.32
UDP-6-deoxy-alpha-D-galactofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.25
UDP-alpha-D-arabinofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.004 - 7.2
UDP-alpha-D-galactofuranose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.33
dTDP-alpha-D-galactofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
42207
0.04
UDP-3-deoxy-alpha-D-threo-hexofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
31085
0.2
UDP-5-deoxy-alpha-D-galactofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
19652
1.17
UDP-6-deoxy-alpha-D-galactofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
28770
0.25
UDP-alpha-D-arabinofuranose
Mycobacterium tuberculosis
-
using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
19653
0.33 - 8.3
UDP-alpha-D-galactofuranose
2320
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
UDP-3''-deoxy-alpha-D-xylo-hexofuranose
-
in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
(2R,3R,4R,5R)-2-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl hydrogen ((E)-((3R,4R,5S)-5-((R)-1,2-dihydroxyethyl)-3,4-dihydroxydihydrofuran-2(3H)-ylidene)fluoromethyl)phosphonate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
0.55
(2R,3R,4R,5R)-2-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl hydrogen ((Z)-((3R,4R,5S)-5-((R)-1,2-dihydroxyethyl)-3,4-dihydroxydihydrofuran-2(3H)-ylidene)fluoromethyl)phosphonate
Mycobacterium tuberculosis
-
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0008
-
using UDP-5-deoxy-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.004
-
using UDP-2''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.006
-
using UDP-5''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.007
-
using UDP-6''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.01
-
using UDP-3-deoxy-alpha-D-threo-hexofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C; using UDP-alpha-D-arabinofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.012
-
using UDP-6-deoxy-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.1
-
using UDP-5-deoxy-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.14
-
using UDP-alpha-D-arabinofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C; using UDP-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.35
-
using dTDP-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
0.9
-
using UDP-6-deoxy-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
1.6
-
using UDP-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37C
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
-
x-ray crystallography
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, free enzyme is crystallized using 5-7% (w/v) PEG 4000, 100 mM sodium cacodylate, pH 6.5, 100 mM sodium acetate. UDP-bound enzyme is crystallized using 6-7% (w/v) PEG 4000, 100 mM sodium acetate, 10% (v/v) glycerol, 5 mM TCEP, at 22C
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, purified enzyme with 20% (v/v) glycerol, four months, no significant loss of activity
-
4C, purified enzyme with 20% (v/v) glycerol, 2 weeks, no significant loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography, gel filtration
-
Ni2+-NTA agarose column chromatography and Sephacryl S-100 gel filtration
-
nickel-Sepharose column chromatography and Sephacryl S-200 gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli C41(DE3) cells
-
expressed in Escherichia coli Rosetta (DE3) Quarters cells
-
expressed in Escherichia coli Tuner (DE3) cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D256A
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
D256E
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
D258A
-
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
D258E
-
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
D372S
-
the mutant shows no detectable activity
E300S
-
the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme
E371S
-
the mutant shows no detectable activity
H413S
-
the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme
W399S
-
the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme
D256A
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
-
D256E
-
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
-
D258A
-
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
-
D258E
-
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
-