Information on EC 2.4.1.287 - rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase

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The expected taxonomic range for this enzyme is: Mycobacterium

EC NUMBER
COMMENTARY hide
2.4.1.287
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RECOMMENDED NAME
GeneOntology No.
rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 2 UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
show the reaction diagram
overall reaction
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UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol = UDP + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
show the reaction diagram
(1a)
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UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol = UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
show the reaction diagram
(1b)
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactofuranose:alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/4-beta-galactofuranosyltransferase (configuration-inverting)
Isolated from Mycobacterium tuberculosis and M. smegmatis, the enzyme has dual beta-(1->4) and beta-(1->5) transferase action. Involved in the formation of the cell wall in mycobacteria.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme is involved in the initial steps of galactan formation gand initiates galactan synthesis on the C50-P-P-GlcNAc-Rha acceptor
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
show the reaction diagram
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
show the reaction diagram
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
show the reaction diagram
additional information
?
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33863
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x * 33863, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Talon resin column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expressed in Mycobacterium smegmatis mc2155 cells
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