Information on EC 2.4.1.280 - N,N'-diacetylchitobiose phosphorylase

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The expected taxonomic range for this enzyme is: Vibrio

EC NUMBER
COMMENTARY hide
2.4.1.280
-
RECOMMENDED NAME
GeneOntology No.
N,N'-diacetylchitobiose phosphorylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N,N'-diacetylchitobiose + phosphate = N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of phosphate
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chitin derivatives degradation
-
-
Amino sugar and nucleotide sugar metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
N,N'-diacetylchitobiose:phosphate N-acetyl-D-glucosaminyltransferase
The enzyme is specific for N,N'-diacetylchitobiose and does not phosphorylate other N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl N,N'-diacetylchitobioside + phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-D-glucosaminide
show the reaction diagram
reaction at 8.5% the rate of N,N'-diacetylchitobiose
-
-
r
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
4-methylumbelliferyl N,N'-diacetylchitobioside + phosphate
show the reaction diagram
reverse phosphorolysis, reaction at 20% the rate of N-acetyl-D-glucosamine
-
-
r
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
4-nitrophenyl N,N'-diacetylchitobioside
show the reaction diagram
reverse phosphorolysis, reaction at 36% the rate of N-acetyl-D-glucosamine
-
-
r
N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
4-nitrophenyl N,N'-diacetylchitobioside + phosphate
show the reaction diagram
reverse phosphorolysis, reaction at 10% the rate of N-acetyl-D-glucosamine
-
-
r
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
N,N'-diacetylchitobiose + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N,N'-diacetylchitobiose + phosphate
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.58 - 2
N,N'-diacetylchitobiose
0.93
N-acetyl-alpha-D-glucosamine 1-phosphate
pH 7.0, 30C, reverse phosphorolysis reaction
1
N-acetyl-D-glucosamine
pH 7.0, 30C, reverse phosphorolysis reaction
0.51
phosphate
pH 7.0, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
89670
calculated from amino acid sequence
90360
-
calculated from amino acid sequence
150000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 90000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystals obtained at 4C using the hanging-drop vapor interactions diffusion method by mixing 5 microl of a protein solution with 5 microl of a reservoir solution comprising 0.1 M HEPES Na-OH (pH 8.0), 0.17 M CaCl2, and 30%-40% (v/v) PEG400
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.1
-
-
714078
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 37
1 h stable
35
-
stable up to
50
1 h 50% loss of activity
70
1 h compete inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli