Information on EC 2.4.1.28 - glucosyl-DNA beta-glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Enterobacteria phage T6

EC NUMBER
COMMENTARY hide
2.4.1.28
-
RECOMMENDED NAME
GeneOntology No.
glucosyl-DNA beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
transfers a beta-D-glucosyl residue from UDP-glucose to a glucosylhydroxymethylcytosine residue in DNA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-glucosyl-DNA beta-D-glucosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9030-15-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Escherichia coli infected with
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + glucosyl-5-hydroxymethylcytosine residue in DNA
UDP + beta-D-glucosyl-beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
show the reaction diagram
-
no transfer of glucose to synthetic 5-hydroxymethylcytosine-DNA or to T6 DNA, enzyme glucosylates glucosylhydroxymethylcytosine residues of either alpha or beta configuration
-
?
UDP-glucose + glucosylhydroxymethylcytosine residue in DNA
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + glucosylhydroxymethylcytosine residue in DNA
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required for activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sulfhydryl reagents
-
required
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0017
-
crude extract
0.0067
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, using streptomycin sulfate treatment and fractionation on DEAE-cellulose
-