Information on EC 2.4.1.279 - nigerose phosphorylase

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The expected taxonomic range for this enzyme is: Lachnoclostridium phytofermentans

EC NUMBER
COMMENTARY hide
2.4.1.279
-
RECOMMENDED NAME
GeneOntology No.
nigerose phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate = D-glucose + beta-D-glucose 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
3-O-alpha-D-glucopyranosyl-D-glucopyranose:phosphate beta-D-glucosyltransferase
The enzymes from Clostridium phytofermentans is specific for nigerose, and shows only 0.5% relative activity with kojibiose (cf. EC 2.4.1.230, kojibiose phosphorylase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate
show the reaction diagram
2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
D-glucose + beta-D-glucose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
D-glucose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
D-glucose + beta-D-glucose 1-phosphate
show the reaction diagram
beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
show the reaction diagram
beta-D-glucose 1-phosphate + D-galactose
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
show the reaction diagram
reverse phosphorolysis reaction 8.5% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose, no significant preference for specific anomer of the acceptor
-
-
?
beta-D-glucose 1-phosphate + D-glucose
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
reverse phosphorolysis reaction, no significant preference for specific anomer of the acceptor
-
-
r
beta-D-glucose 1-phosphate + D-xylose
3-O-alpha-D-glucopyranosyl-D-xylopyranose + 2-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
show the reaction diagram
reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
products are formed in the ratio 63:37%
-
?
beta-D-glucose 1-phosphate + methyl alpha-D-glucoside
methyl-3-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + methyl-2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate
show the reaction diagram
reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
products are formed in the ratio 71:29%
-
?
D-galactose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
show the reaction diagram
D-glucose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
D-xylose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
show the reaction diagram
38% activity compared to D-glucose as acceptor
-
-
r
methyl alpha-D-glucopyranoside + beta-D-glucopyranose 1-phosphate
methyl 3-O-alpha-D-glucopyranosyl-D-glucopyranoside + phosphate
show the reaction diagram
2.1% activity compared to D-glucose as acceptor
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
D-glucose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
D-glucose + beta-D-glucose 1-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-O-alpha-D-glucopyranosyl-D-glucopyranose
-
Co2+
1 mM, 80% inhibition
Cu2+
1 mM, 98.7% inhibition
Fe2+
1 mM, 68% inhibition
Ni2+
1 mM, 95.4% inhibition
Zn2+
1 mM, 85% inhibition
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 79
1,5-Anhydro-D-glucitol
1.7
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C, phosphorolyis reaction
1.2
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
20 - 104
D-galactose
2.1 - 3.3
D-glucose
1 - 86
D-xylose
79
methyl alpha-D-glucopyranoside
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
0.2 - 0.49
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30 - 120
1,5-Anhydro-D-glucitol
31
3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL5
pH 7.0, 30C
58
beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
A9KSL5
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
25 - 69
D-galactose
52 - 110
D-glucose
40 - 118
D-xylose
8.3
methyl alpha-D-glucopyranoside
Lachnoclostridium phytofermentans
A9KSL5
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
67
phosphate
Lachnoclostridium phytofermentans
A9KT32
pH 7.0, 30C, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5 - 1.8
1,5-Anhydro-D-glucitol
2926
49
beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
A9KSL5
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
194820
0.7 - 1.3
D-galactose
71
24 - 33
D-glucose
35
1.4 - 2.4
D-xylose
115
0.11
methyl alpha-D-glucopyranoside
Lachnoclostridium phytofermentans
A9KSL5
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
7494
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.4
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.17
pH 7.0, 30C, phosphorolysis reaction, substrate 2-O-alpha-D-glucopyranosyl-D-glucopyranose; pH 7.0, 30C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + methyl alpha-D-glucoside
4.6
pH 7.0, 30C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-galactose
8.1
pH 7.0, 30C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
9.3
pH 7.0, 30C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-xylose
32
pH 7.0, 30C, phosphorolysis reaction, substrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
54
pH 7.0, 30C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-glucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
87170
calculated from amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21
expression in Escherichia coli