Information on EC 2.4.1.275 - neolactotriaosylceramide beta-1,4-galactosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.275
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RECOMMENDED NAME
GeneOntology No.
neolactotriaosylceramide beta-1,4-galactosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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neolacto-series glycosphingolipids biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactose:N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1beta4)-beta-D-glucosyl-(1<->1)-ceramide 4-beta-D-galactosyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + 1-O-benzyl 6-O-beta-D-GlcpNAC-beta-D-GlypNAc
UDP + 1-O-benzyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-beta-D-GlcpNAc
show the reaction diagram
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?
UDP-alpha-D-galactose + 1-O-benzyl beta-D-GlcpNAc
UDP + 1-O-benzyl 4-O-beta-D-Galp-beta-D-GlcpNAc
show the reaction diagram
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no activity with alpha-D-GlcNAc-1-benzyl
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?
UDP-alpha-D-galactose + 1-O-methyl 3-O-beta-D-GlcpNAc-beta-D-Galp
UDP + 1-O-methyl 4-O-beta-D-Galp-3-O-beta-D-GlcpNAc-beta-D-Galp
show the reaction diagram
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?
UDP-alpha-D-galactose + 4-nitrophenyl N-acetyl-1-thio-beta-D-glucosaminide
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosiy-N-acetyl-1-thio-beta-D-glucosaminide
show the reaction diagram
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?
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
UDP-galactose + 1-O-(1-methylumbellifer-7-yl) beta-D-GlcpNAc
UDP + 1-O-(1-methylumbellifer-7-yl) 4-O-beta-D-Galp-beta-D-GlcpNAc
show the reaction diagram
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?
UDP-galactose + 1-O-(4-nitrophenyl) beta-D-GlcpNAc
UDP + 1-O-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-GlcpNAc
show the reaction diagram
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?
UDP-galactose + 1-O-methyl 6-O-beta-D-GlcpNAC-alpha-D-Manp
UDP + 1-O-methyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-alpha-D-Manp
show the reaction diagram
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?
UDP-galactose + 1-S-(4-nitrophenyl) beta-D-1-thio-GlcpNAc
UDP + 1-S-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-1-thio-GlcpNAc
show the reaction diagram
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?
UDP-galactose + beta-D-GlcpNAc-(1->6)-D-GlcpNAc
UDP + beta-D-Galp-(1->4)-[beta-D-GlcpNAc-(1->6)]-D-GlcpNAc
show the reaction diagram
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UDP-galactose + GlcNAc-beta-(1->6)-(GlcNAc-beta-(1->2))-Man-alpha-(1->3)-Man-beta-(1->)O(CH2)8COOCH2-m-nitrophenol
UDP + Gal-alpha-(1->4)-GlcNAc-beta-(1->6)-(GlcNAc-beta-(1->2))-Man-alpha-(1->3)-Man-beta-(1->)O(CH2)8COOCH2-m-nitrophenol
show the reaction diagram
preferential galactosylation of the GlcNAc-beta-(1->6)-Man branch
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UDP-galactose + N,N',N''-triacetylchitotriose
UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
show the reaction diagram
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?
UDP-galactose + N,N'-diacetylchitobiose
UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+
Fe3+
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Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+
Mg2+
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Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+
Mn2+
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Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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completely inhibited by addition of EDTA
additional information
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the enzyme is not inhibited at high concentrations of either benzyl-beta-D-N-acetyl-D-glucosamine or free N-acetylglucosamine
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-lactalbumin
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the enzyme shows a marked increase in N-acetyllactosamine synthase activity in the presence of alpha-lactalbumin. Two-fold activation is achieved at 0.25 mg/ml and almost 8fold at 20 mg/ml
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.238
4-nitrophenyl N-acetyl-1-thio-beta-D-glucosaminide
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pH 7.0, temperature not specified in the publication
0.11
N-acetyl-beta-D-galactosaminopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->)-ceramide
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pH 7.2, 37°C
0.105 - 0.25
UDP-galactose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.344
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pH 7.2, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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the specific activities of Gal-Tr is nearly normal or slightly elevated in all the three Purkinje cell abnormality mutants
Manually annotated by BRENDA team
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specific activities of Gal-Tr in the granule cell layer and white matter is approximately 66% of that in the molecular layer. Gal-Tr in the homogenate of cerebellum declines by approximately 35-45% from postnatal day 3 to 14. Gal-Tr activity in the granule cells does not change with age. The relative Gal-Tr activity in granule cells to homogenate is between 0.13 and 0.23
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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mineral oil induced
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme is partially stabilized in the presence of phospholipid liposomes
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the purified GalT-4 is very unstable and loses its enzymatic activity within 48 h. The enzymatic activity could be partially stabilized in the presence of N-acetylglucosamine and Mn2+, at least for 2 weeks at 4°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in insect cells
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expression in Sf-9 cells
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