Information on EC 2.4.1.271 - crocetin glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.271
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RECOMMENDED NAME
GeneOntology No.
crocetin glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
(3)
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UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + bis(beta-D-glucosyl) crocetin
show the reaction diagram
(2)
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UDP-alpha-D-glucose + crocetin = UDP + beta-D-glucosyl crocetin
show the reaction diagram
(1)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
crocetin esters biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:crocetin 8-O-D-glucosyltransferase
In the plants Crocus sativus and Gardenia jasminoides this enzyme esterifies a free carboxyl group of crocetin and some crocetin glycosyl esters. The enzyme from Gardenia can also form glucosyl esters with 4-coumarate, caffeate and ferulate [3].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
UDP-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
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?
UDP-glucose + beta-D-glucosyl crocetin
UDP + bis(beta-D-glucosyl) crocetin
show the reaction diagram
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-
-
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?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
show the reaction diagram
additional information
?
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the enzyme does not add other glucose units to the glucosyl-esters to form crocetin gentiobiosyl-esters
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
UDP-glucose + beta-D-gentiobiosyl crocetin
UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
show the reaction diagram
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?
UDP-glucose + beta-D-glucosyl crocetin
UDP + bis(beta-D-glucosyl) crocetin
show the reaction diagram
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-
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?
UDP-glucose + crocetin
UDP + beta-D-glucosyl crocetin
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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10 mM, 69% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
crocetin
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pH 7.1, 40C
0.72
UDP-glucose
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pH 7.1, 40C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.6
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pH 7.1, 40C
10.8
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pH 7.1, 40C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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assay at
7.1
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assay at
7.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
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35C: about 75% of maximal activity, 45C: about 85% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.4 - 4.6
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the 300fold purified extract is subjected to isoelectric focusing and gives three active GTASE1 bands at pH 4.4, 4.5, and 4.6
additional information
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the most purified preparation shows isoforms between pH 4.4 and 4.8 when resolved by isoelectric focusing
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
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2 * 26000, SDS-PAGE
47000
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gel filtration
49000 - 55000
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gel filtrration
50700
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x * 50700, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 26000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified 125fold trough a multi-step process of acetone fractionation, anion-exchange, and gel filtration chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as His-tag fusion protein in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
GTase1 activity is higher during the first four days of crocin glucosides biosynthesis, but decreases after four days
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treatment with jasmonic acid or salicylic acid, drough, wounding and dehydration fail to affect the expression levels of UGTCs2
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