Information on EC 2.4.1.263 - abscisate beta-glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.263
-
RECOMMENDED NAME
GeneOntology No.
abscisate beta-glucosyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-D-glucose + abscisate = UDP + beta-D-glucopyranosyl abscisate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
abscisic acid degradation by glucosylation
-
-
Biosynthesis of secondary metabolites
-
-
Carotenoid biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-D-glucose:abscisate beta-D-glucosyltransferase
The enzyme acts better on (S)-2-trans-abscisate than the natural (S)-2-cis isomer, abscisate, or its enantiomer, the (R)-2-cis isomer.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
adzuki bean
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose + (+)-(S)-abscisic acid
UDP + (S)-abscisic acid beta-D-glucosyl ester
show the reaction diagram
-
-
-
?
UDP-D-glucose + (-)-R-abscisic acid
UDP + abscisic acid D-glucosyl ester
show the reaction diagram
-
-
-
?
UDP-D-glucose + 2-trans-(+)-abscisic acid
UDP + abscisic acid D-glucosyl ester
show the reaction diagram
the recombinant GTase converts 2-trans-(+)-abscisic acid better than (+)-S-abscisic acid and (-)-R-abscisic acid
-
-
?
UDP-D-glucose + abscisic acid
UDP + abscisic acid D-glucosyl ester
show the reaction diagram
-
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
abscisic acid
-
apparent value, at pH 5.0, temperature not specified in the publication
8
UDP-D-glucose
-
apparent value, at pH 5.0, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
in phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
glutathione S-transferase-GTase fusion protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
none of the plant hormones, GA3, IAA, zeatin, or jasmonic acid, significantly promotes the gene expression
the mRNA level is increased by absicsic acid application. Drought and wounding treatments rapidly increase the level of the AOG transcript. The drought treatment slightly increases the mRNA level within 1 h reaching the maximum within 6 h. The wounding treatment increases the expression of the AOG gene, even more rapidly than the drought treatment within 30 min reaching the maximum after 6 h