Information on EC 2.4.1.263 - abscisate beta-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
2.4.1.263
-
top print hide
RECOMMENDED NAME
GeneOntology No.
abscisate beta-glucosyltransferase
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-D-glucose + abscisate = UDP + beta-D-glucopyranosyl abscisate
show the reaction diagram
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
abscisic acid degradation by glucosylation
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Carotenoid biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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-
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-glucose:abscisate beta-D-glucosyltransferase
The enzyme acts better on (S)-2-trans-abscisate than the natural (S)-2-cis isomer, abscisate, or its enantiomer, the (R)-2-cis isomer.
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top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
adzuki bean
UniProt
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose + (+)-(S)-abscisic acid
UDP + (S)-abscisic acid beta-D-glucosyl ester
show the reaction diagram
-
-
-
?
UDP-D-glucose + (-)-R-abscisic acid
UDP + abscisic acid D-glucosyl ester
show the reaction diagram
-
-
-
?
UDP-D-glucose + 2-trans-(+)-abscisic acid
UDP + abscisic acid D-glucosyl ester
show the reaction diagram
the recombinant GTase converts 2-trans-(+)-abscisic acid better than (+)-S-abscisic acid and (-)-R-abscisic acid
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-
?
UDP-D-glucose + abscisic acid
UDP + abscisic acid D-glucosyl ester
show the reaction diagram
-
-
-
-
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
abscisic acid
-
apparent value, at pH 5.0, temperature not specified in the publication
8
UDP-D-glucose
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apparent value, at pH 5.0, temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
in phosphate buffer
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
glutathione S-transferase-GTase fusion protein
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
none of the plant hormones, GA3, IAA, zeatin, or jasmonic acid, significantly promotes the gene expression
the mRNA level is increased by absicsic acid application. Drought and wounding treatments rapidly increase the level of the AOG transcript. The drought treatment slightly increases the mRNA level within 1 h reaching the maximum within 6 h. The wounding treatment increases the expression of the AOG gene, even more rapidly than the drought treatment within 30 min reaching the maximum after 6 h
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.263)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)