Information on EC 2.4.1.262 - soyasapogenol glucuronosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.262
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RECOMMENDED NAME
GeneOntology No.
soyasapogenol glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucuronate + soyasapogenol B = UDP + soyasapogenol B 3-O-D-glucuronide
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
soybean saponin I biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-glucuronate:soyasapogenol 3-O-D-glucuronosyltransferase
Requires a divalent ion, Mg2+ better than Mn2+, better than Ca2+. Also acts on soysapogenol A and E.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucuronate + soyasapogenol A
UDP + soyasapogenol A 3-O-D-glucuronide
show the reaction diagram
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40.6% activity compared to soyasapogenol B
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?
UDP-D-glucuronate + soyasapogenol B
UDP + soyasapogenol B 3-O-D-glucuronide
show the reaction diagram
UDP-D-glucuronate + soyasapogenol E
UDP + soyasapogenol E 3-O-D-glucuronide
show the reaction diagram
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79.08% activity compared to soyasapogenol B
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?
UDP-galactose + soyasapogenol B
?
show the reaction diagram
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8.35% activity compared to UDP-D-glucuronate
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?
UDP-glucose + soyasapogenol B
?
show the reaction diagram
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7.94% activity compared to UDP-D_glucuronate
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?
additional information
?
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beta-amyrin, sophoradiol, and glycyrrhetinic acid are not converted by UGASGT. UGASGT does not catalyze the transfer reaction of glucuronate to soyasapogenol B-3-O-beta-glucuronide
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+
Mg2+
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the optimum concentration of Mg2+ is between 10 and 25 mM. UGASGT shows an absolute requirement for divalent cations. The effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+
Mn2+
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the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+
additional information
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Na+, K+, Co2+, Fe2+, Zn2+, Cu2+, Al3+, and Fe3+, activate UGASGT only slightly or not at all
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mega-10
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the detergent inactivates UGASGT during solubilization
SM-1000
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the detergent inactivates UGASGT during solubilization
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00403
soyasapogenol A
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in 100 mM Tris-HCl (pH 8.0), at 40°C
0.01722
soyasapogenol B
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in 100 mM Tris-HCl (pH 8.0), at 40°C
0.04194
soyasapogenol E
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in 100 mM Tris-HCl (pH 8.0), at 40°C
0.02394
UDP-D-glucuronate
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using soyasapogenol B as the sugar acceptor, in 100 mM Tris-HCl (pH 8.0), at 40°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.342
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crude extract, in 100 mM Tris-HCl (pH 8.0), at 40°C
7
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after 204.68fold purification, in 100 mM Tris-HCl (pH 8.0), at 40°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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in Tris-HCl buffer (pH 8.0)
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE
66000
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the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
CHAPS > deoxy-BIGCHAP > n-octyl-beta-D-thioglucoside. Sodium cholate, SM-1200, and n-dodecyl-beta-D-maltoside do not effectively solubilize UGASGT
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the effectiveness of detergents at the CMC in solubilizing UGASGT is as follows: MEGA10 > SM-1000 >
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HiTrap Q column chromatography, Superdex 200 gel filtration, and HiTrap Blue column chromatography column chromatography
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
UDP-D-glucuronic acid: soyasapogenol B glucuronosyltransferase activity in the methyl jasmonate-treated cells is 2times higher than that in the control cells
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