Information on EC 2.4.1.253 - baicalein 7-O-glucuronosyltransferase

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The expected taxonomic range for this enzyme is: Scutellaria baicalensis

EC NUMBER
COMMENTARY hide
2.4.1.253
-
RECOMMENDED NAME
GeneOntology No.
baicalein 7-O-glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-D-glucuronate + baicalein = UDP + baicalin
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
baicalein metabolism
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wogonin metabolism
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-glucuronate:5,6,7-trihydroxyflavone 7-O-glucuronosyltransferase
The enzyme is specific for UDP-D-glucuronate as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucuronate + 4',5,6,7-tetrahydroxyflavone
UDP + 4',5,6,7-tetrahydroxyflavone 7-O-beta-D-glucuronate
show the reaction diagram
UDP-D-glucuronate + 5,6,7-trihydroxyflavone
UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate
show the reaction diagram
UDP-D-glucuronate + 5,7-dihydroxy-8-methoxyflavone
UDP + 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucoronate
show the reaction diagram
UDP-glucuronate + baicalein
UDP + baicalin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-glucuronate + 5,6,7-trihydroxyflavone
UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate
show the reaction diagram
UDP-glucuronate + baicalein
UDP + baicalin
show the reaction diagram
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-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no divalent cation requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4,4'-diisothiocyanostilbene-2,2'-disulfonic acid
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0.1 mM, complete inhibition
CuSO4
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1 mM, complete inhibition
Diethylpyrocarbonate
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0.1 mM, 31% inhibition
EDTA
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5 mM, 17% inhibition
FeCl2
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1 mM, complete inhibition
MgCl2
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1 mM, 21.5% inhibition
p-chloromercuribenzoate
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0.1 mM, complete inhibition
ZnCl2
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1 mM, complete inhibition
additional information
-
N-ethylmaleimide has no effect in activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0524
5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one
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pH 7.5, 37°C
0.109
scutellarein
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pH 7.5, 37°C
0.0729
UDP-glucuronate
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pH 7.5, 37°C
0.107
wogonin
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pH 7.5, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Scutellaria baicalensis
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Vmax/Km ratio for 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one (i.e. baicalein) is: 4.9 nkat/mg*micromol, Vmax/Km ratio for scutellarein is 2.67 nkat/mg*micromol, Vmax/Km ratio for wogonin is: 1.71 nkat/mg*micromol
2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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in 50 mM Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8
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chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
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2 * 52000, does not exist or function as monomer, SDS-PAGE
110000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
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50% of the activity remains after 3 h, 25% of the activity remains after 24 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for more than 2 weeks without loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
high temperature of 40°C increases the level of UDP-glucuronate:baicalein 7-O-glucuronosyltransferase transcripts, whereas low temperature does not affect the level of these transcripts compared with their level at 30°C. At 40°C, the expression after 40-days exposure is significantly higher than that after 30 days
the gene encoding UBGAT shows an expression peak in August
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