Information on EC 2.4.1.244 - N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase

Word Map on EC 2.4.1.244
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.244
-
RECOMMENDED NAME
GeneOntology No.
N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-beta-D-glucosaminyl group = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Various types of N-glycan biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:N-acetyl-beta-D-glucosaminyl-group 4-beta-N-acetylgalactosaminyltransferase
The enzyme from human can transfer N-acetyl-D-galactosamine (GalNAc) to N-glycan and O-glycan substrates that have N-acetyl-D-glucosamine (GlcNAc) but not D-glucuronic acid (GlcUA) at their non-reducing end. The N-acetyl-beta-D-glucosaminyl group is normally on a core oligosaccharide although benzyl glycosides have been used in enzyme-characterization experiments. Some glycohormones, e.g. lutropin and thyrotropin contain the N-glycan structure containing the N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group.
CAS REGISTRY NUMBER
COMMENTARY hide
163913-55-1
-
67338-98-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
B4GALNT3 overexpression enhances colon cancer cell malignant phenotypes in vitro and in vivo. B4GALNT3 knockdown suppresses EGF-induced phosphorylation of epithelial growth factor receptor, EGFR, and its downstream signaling molecules. B4GALNT3 knockdown inhibits EGFR activity, decreases EGF-induced migration and invasion in colon cancer cells, and promotes EGFR degradation in colon cancer cells, overview
physiological function
beta1,4-N-acetylgalactosaminyltransferase III modulates cancer stemness through EGFR signaling pathway in colon cancer cells, B4GALNT3 regulates cancer stemness and the invasive properties of colon cancer cells through modifying EGFR glycosylation and signaling. B4GALNT3 expression is positively correlated with advanced stages and poor survival in colorectal cancer patients
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-GalNAc + Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
UDP + Gal-beta-(1,4)-GalNAc-beta(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
show the reaction diagram
UDP-GalNAc + Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-2-pyridylaminoside
UDP + Gal-beta-(1,4)-GalNAc-beta(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
show the reaction diagram
UDP-GalNAc + GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
UDP + GalNAc-beta(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
show the reaction diagram
UDP-GalNAc + GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-2-pyridylaminoside
UDP + GalNAc-beta(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
show the reaction diagram
UDP-GalNAc + GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-2-pyridylaminoside
UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[Gal-beta-(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
UDP + GalNAc-beta(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
show the reaction diagram
UDP-GalNAc + GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-[Fuc-alpha-(1,6)]-GlcNAc-2-pyridylaminoside
UDP + GalNAc-beta(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside
show the reaction diagram
UDP-GalNAc + GlcNAc-beta-(1,3)-GalNAc-alpha-p-nitrophenyl
UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,3)-GalNAc-alpha-p-nitrophenyl
show the reaction diagram
UDP-GalNAc + GlcNAc-beta-(1,3)Fuc-dansyl
UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,3)Fuc-dansyl
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + GlcNAc-beta-(1,6)-GalNAc-alpha-p-nitrophenyl
UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,6)-GalNAc-alpha-p-nitrophenyl
show the reaction diagram
190.7% of the activity with GlcNAcbeta-O-benzyl
-
-
?
UDP-GalNAc + GlcNAc-beta-(1,6)[Gal-beta-(1,3)]GalNAc-alpha-p-nitrophenyl
UDP + GalNAc-beta-(1,4)-GlcNAc-beta-(1,6)[Gal-beta-(1,3)]GalNAc-alpha-p-nitrophenyl
show the reaction diagram
15.2% of the activity with GlcNAcbeta-O-benzyl
-
-
?
UDP-GalNAc + GlcNAc-p-nitrophenyl
?
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + GlcNAcbeta-O-benzyl
UDP + GalNAc-beta-(1,4)-GlcNAcbeta-O-benzyl
show the reaction diagram
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-beta-D-glucosamine
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosamine
show the reaction diagram
enzyme B4GALNT3 transfers N-acetylgalactosamine (GalNAc) to the non-reducing N-acetylglucosamine (GlcNAc) to form GalNAcbeta1-4GlcNAc (LacdiNAc) in an in vitro enzymatic assay
-
-
-
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-beta-D-glucosaminyl group
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + GlcNAcbeta1,2Manalpha1,6[GlcNAcbeta1,2Manalpha1,3]Manbeta1,4GlcNAcbeta1,4GlcNAc-aminopyridine
?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + GlcNAcbeta1,2Manalpha1,6[GlcNAcbeta1,4[GlcNAcbeta1,2]Manalpha1,3]Manbeta1,4GlcNAcbeta1,4GlcNAc-aminopyridine
?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + GlcNAcbeta1,3Fuc-dansyl
?
show the reaction diagram
-
GalNAc is efficiently transferred toward N-glycans, O-glycans, and the arthro-series glycosphingolipids
-
-
?
UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl-R group
UDP + N-acetyl-beta-D-galactosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl group
show the reaction diagram
-
beta4GalNAcTA contributs to the synthesis of the GalNAcbeta1,4GlcNA structure in vivo and may play a role in the physiological or neuronal development of Drosophila
-
-
?
UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl-R group
UDP + N-acetyl-beta-D-galactosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl-R group
show the reaction diagram
-
beta4Gal-NAcTA has at least three distinct, tissue-specific roles in neurons and muscles. Substrates are present in both neural and muscle cells that require glycosylation by beta4GalNAcTA for normal function
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl-R group
UDP + N-acetyl-beta-D-galactosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl group
show the reaction diagram
-
beta4GalNAcTA contributs to the synthesis of the GalNAcbeta1,4GlcNA structure in vivo and may play a role in the physiological or neuronal development of Drosophila
-
-
?
UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl-R group
UDP + N-acetyl-beta-D-galactosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl-R group
show the reaction diagram
-
beta4Gal-NAcTA has at least three distinct, tissue-specific roles in neurons and muscles. Substrates are present in both neural and muscle cells that require glycosylation by beta4GalNAcTA for normal function
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
beta4GalNAcT-II acts as a negative regulator of carbohydrate determinant sialyl Lewis x expression in colon cancer
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta4Gal-NAcTB pilot protein
-
generates a functionally active beta4GalNAcTB, in the absence of beta4Gal-NAcTB pilot protein inactive beta4GalNAcTB is trapped in the endoplasmic reticulum
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
primary, B4GALNT3 expression in different stage of colorectal tumors, overview
Manually annotated by BRENDA team
-
beta4GalNAcT mRNA is expressed throughout the life cycle of the fly, i.e., from the embryo stage. In the larval stage, mRNA is expressed to a greater extent in the eye disc and larval brain than in the wing disc and leg disc
Manually annotated by BRENDA team
high enzyme expression level in epithelial cells of gastric mucosa
Manually annotated by BRENDA team
-
beta4Gal-NAcTA has at least three distinct, tissue-specific roles in neurons and muscles. Substrates are present in both neural and muscle cells that require glycosylation by beta4GalNAcTA for normal function
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anti-FLAG M1 affinity gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in HCT-116 cells
-
expressed in in HEK-293T cells
-
expressed in LS-174T cells
-
expressed in S2 cells
-
expressed in sf21 cells
-
full-length cDNA is cloned into vectors for expression in cultured Drosophila S2; full-length cDNA is cloned into vectors for expression in cultured Drosophila S2
gene B4GALNT3
the putative catalytic domain of beta4GalNAc-T4 is expressed as a secreted protein fused with a FLAG peptide in HEK293T cells
-
truncated form of the human enzyme is expressed in HEK293T cells as a soluble protein
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of B4GALNT3 is regulated in colonospheres
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
B4GALNT3 overexpression enhances colon cancer cell malignant phenotypes in vitro and in vivo. Knockdown of B4GALNT3 using small interfering (si) RNAs in colon cancer cell lines (HCT116, SW480, HCT15, and HT29 cells) decrease sphere formation and the expression of stem cell markers, OCT4 and. NANOG. B4GALNT3 knockdown suppresses EGF-induced phosphorylation of epithelial growth factor receptor, EGFR, and its downstream signaling molecules
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
GalNAC-beta-(1,4)-GlcNAc and beta4GalNAc-T3 are novel differentiation markers of surface mucous cells in the gastric mucosa