Information on EC 2.4.1.239 - flavonol-3-O-glucoside glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.239
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RECOMMENDED NAME
GeneOntology No.
flavonol-3-O-glucoside glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of glycosyl group
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-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
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kaempferol glycoside biosynthesis (Arabidopsis)
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kaempferol triglucoside biosynthesis
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quercetin glycoside biosynthesis (Arabidopsis)
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quercetin triglucoside biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:flavonol-3-O-beta-D-glucoside 2''-O-beta-D-glucosyltransferase
One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-18-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cultivars Nezumisaya and Harosoy, gene Fg3
UniProt
Manually annotated by BRENDA team
var. Alaska
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
glycosyltransferases are classified into at least 96 families, the family 1 glycosyltransferase, referred to as UDP glycosyltransferases (UGTs), comprise the largest group in plants. UGTs catalyze the transfer of a glycosyl moiety from UDP sugars to a wide range of acceptor molecules including flavonoids
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
TDP-glucose + a flavonol 3-O-beta-D-glucoside
TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
show the reaction diagram
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3fold lower rate than with UDP-glucose as glucosyl donor
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-
?
UDP-glucose + a flavonol 3-O-beta-D-glucoside
UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
show the reaction diagram
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highly specific enzyme, specific for flavonol-3-O-monoglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor
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-
?
UDP-glucose + a flavonol 3-O-beta-D-glucoside
UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside
show the reaction diagram
-
-
-
?
UDP-glucose + kaempferol 3-O-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
show the reaction diagram
UDP-glucose + kaempferol 3-O-glucoside
UDP + kaempferol 3-O-sophoroside
show the reaction diagram
activity of the enzyme from cultivar Harosoy
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-
?
UDP-glucose + myricetin 3-O-beta-D-glucoside
UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
show the reaction diagram
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50% of the activity with kaempferol 3-O-beta-D-glucoside
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-
?
UDP-glucose + pelargonidin 3-O-beta-D-glucoside
UDP + pelargonidin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
show the reaction diagram
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2% of the activity with kaempferol 3-O-beta-D-glucoside
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-
?
UDP-glucose + quercetin 3-O-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactor requirement
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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not activated by divalent cations, 0.1-10 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
UDP
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product inhibition, 1.75 mM, 50% inhibition
additional information
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not inhibited by divalent cations, 0.1-10 mM, EDTA, EGTA, no substrate inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
kaempferol 3-O-beta-D-glucoside
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pH 7.3, 30C
0.64
UDP-glucose
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pH 7.3, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
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assay at
8 - 9
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0.2 M glycine-NaOH buffer, lower activity in 0.2 M Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C or room temperature, partially purified enzyme, 36 h, 80% loss of activity
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4C, 2 weeks, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
331fold, separation of three distinct flavonol:glucosyltransferases
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene Fg3 or GmF3G2''Gt in the molecular linkage group C2, chromosome 6, DNA and amino acid sequence determination and analysis, genotyping, unrooted molecular phylogenetic tree
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information