Information on EC 2.4.1.223 - glucuronosyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.223
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RECOMMENDED NAME
GeneOntology No.
glucuronosyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1-A>3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transferase
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosaminoglycan biosynthesis - heparan sulfate / heparin
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heparan sulfate biosynthesis (late stages)
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine 4IV-alpha-N-acetyl-D-glucosaminyltransferase (configuration-retaining)
Enzyme involved in the initiation of heparin and heparan sulfate synthesis, transferring GlcNAc to the (GlcA-Gal-Gal-Xyl-)Ser core. Apparently products of both the human EXTL2 and EXTL3 genes can catalyse this reaction. In Caenorhabditis elegans, the product of the rib-2 gene displays this activity as well as that of EC 2.4.1.224, glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase. For explanation of the use of a superscript in the systematic name, see {iupac/2carb/37#372::2-Carb-37.2}.
CAS REGISTRY NUMBER
COMMENTARY hide
145539-84-0
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336193-98-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene rib-2
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-1-O-Ser
UDP + alpha-N-acetyl-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-1-O-Ser
show the reaction diagram
UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-naphthalenemethanol
UDP + alpha-N-acetyl-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl1-O-naphthalenemethanol
show the reaction diagram
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?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1-3)-beta-D-N-acetylgalactosamine
UDP + alpha-N-acetyl-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-N-acetylgalactosamine
show the reaction diagram
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UDP-N-acetyl-D-glucosamine + alpha-thrombomodulin
UDP + alpha-N-acetyl-D-glucosaminyl-alpha-thrombomodulin
show the reaction diagram
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55% of the activity with beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-beta-O-C2H4NHCbz
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?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-1-beta-O-Ser-Gly-Trp-Pro-Asp-Gly
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-1-beta-O-Ser-Gly-Trp-Pro-Asp-Gly
show the reaction diagram
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18% of the activity with beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-beta-O-C2H4NHCbz
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?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-beta-O-C2H4NHCbz
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-beta-O-C2H4NHCbz
show the reaction diagram
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UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-naphthalenemethanol
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl1-O-naphthalenemethanol
show the reaction diagram
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28% of the rate with UDP-N-acetyl-D-galactosamine
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?
UDP-N-acetyl-D-glucosamine + glypican-1
UDP + alpha-N-acetyl-D-glucosaminyl-glypican-1
show the reaction diagram
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30% of the activity with beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-beta-O-C2H4NHCbz
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?
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
show the reaction diagram
additional information
?
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Q6PA81
N-acetylgalactosaminyltransferase-like 1 specifically interferes with the activity of TGF-beta type II receptor ActR-IIB in vivo
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
benzyl-N-acetyl-D-galactosamine
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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fetal liver
Manually annotated by BRENDA team
additional information
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ubiquitous expression of gene
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme lacking first N-terminal 57 amino acids to give a soluble protein, expression in malignant fibrous histiocytoma cell line
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Cos-1 cells
expression in COS-1 cells, lacking the putative NH2-terminal transmembrane and cytoplasmic domains
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expression in COS-1 cells, truncated form , lacking the first 58 N-terminal amino acids
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information