Information on EC 2.4.1.215 - cis-zeatin O-beta-D-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.215
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RECOMMENDED NAME
GeneOntology No.
cis-zeatin O-beta-D-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + cis-zeatin = UDP + O-beta-D-glucosyl-cis-zeatin
show the reaction diagram
; The enzyme from maize can use cis-zeatin and UDPglucose as substrates, but not cis-ribosylzeatin, trans-zeatin or trans-ribosylzeatin. Unlike EC 2.4.1.203, trans-zeatin O-beta-D-glucosyltransferase, UDPxylose cannot act as a donor
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of hexosyl group
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cytokinin-O-glucosides biosynthesis
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Zeatin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:cis-zeatin O-beta-D-glucosyltransferase
The enzyme from maize can use cis-zeatin and UDP-glucose as substrates, but not cis-ribosylzeatin, trans-zeatin or trans-ribosylzeatin. Unlike EC 2.4.1.203, trans-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose cannot act as a donor.
CAS REGISTRY NUMBER
COMMENTARY hide
123644-76-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
cZOGT3 overexpressing lines do not show shortened shoots; transgenic rice lines ectopically overexpressing the cZOGT1 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number; transgenic rice lines ectopically overexpressing the cZOGT2 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number
physiological function
cis-zeatin inhibits seminal root elongation and up-regulates cytokinin-inducible genes, and its activities are comparable to those of trans-zeatin. cis-Zeatin activity has a physiological impact on the growth and development of rice; cis-zeatin inhibits seminal root elongation and up-regulates cytokinin-inducible genes, and its activities are comparable to those of trans-zeatin. cis-Zeatin activity has a physiological impact on the growth and development of rice; cis-zeatin inhibits seminal root elongation and up-regulates cytokinin-inducible genes, and its activities are comparable to those of trans-zeatin. cis-Zeatin activity has a physiological impact on the growth and development of rice
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + cis-zeatin
UDP + O-beta-D-glucosyl-cis-zeatin
show the reaction diagram
UDP-glucose + o-hydroxythidiazuron
UDP + ?
show the reaction diagram
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128% of the activity with trans-zeatin
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?
UDP-glucose + o-topolin
UDP + O-beta-D-glucosyl-o-topolin
show the reaction diagram
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490% of the activity with cis-zeatin
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + cis-zeatin
UDP + O-beta-D-glucosyl-cis-zeatin
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
cZOGTs catalyze O-glucosylation in a Mg2+-dependent manner; cZOGTs catalyze O-glucosylation in a Mg2+-dependent manner; cZOGTs catalyze O-glucosylation in a Mg2+-dependent manner
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
benzyladenine
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kinetin
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N6-(DELTA2-isopentenyl)adenine
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thidiazuron
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trans-zeatin
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.046 - 0.33
cis-zeatin
0.18
o-topolin
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0.11
UDP-glucose
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cisZOG1
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 0.9
cis-zeatin
0.03
o-topolin
Zea mays
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3 - 4.7
cis-zeatin
3679
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
assay at; assay at; assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
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theoretical value
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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lower level of mRNA
Manually annotated by BRENDA team
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high expression of cisZOG1
Manually annotated by BRENDA team
additional information
OscZOG1 is preferentially expressed in shoot and root meristematic tissues and nascent organs
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51100
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estimated from gene sequence
52000
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52200
x * 52200, cZOGT3, SDS-PAGE
55100
x * 55100, cZOGT1, SDS-PAGE
55400
x * 55400, cZOGT2, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 52200, cZOGT3, SDS-PAGE; x * 55100, cZOGT1, SDS-PAGE; x * 55400, cZOGT2, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cZOGT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression profiles of cZOGT genes in rice, overview; cZOGT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression profiles of cZOGT genes in rice, overview; cZOGT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression profiles of cZOGT genes in rice, overview
expression in Escherichia coli
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gene OscZOG1, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme OscZOG1 in Oryza sativa plants using Agrobacterium tumefaciens strain GV3101 transfection
transgenic Zea mays plants are obtained using an Agrobacterium mediated transformation procedure, Ubi : ZOG1 maize line overexpressing the ZOG1 gene of Phaseolus lunatus display increased levels of zeatin-O-glucoside, zeatin O-glucosylation affects root formation, leaf development, chlorophyll content, senescence, male flower differentiation and the formation of tasselseed
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
construction of enzyme OscZOG1-overexpression transgenic lines and knockout lines, phenotypes, overview. O-glucosides of cis-zeatin (cZOG) are increased significantly in shoots of the OscZOG1-overexpression transgenic rice line (OE-3) while no significant differences are found in the levels of transzeatin O-glucosides (tZOG) between wild-type and the overexpression transgenic line OE-3. In contrast, the amounts of cZOG are lower in shoots of the OscZOG1-RNAi transgenic line (Ri-1) comapred to the wild-type shoots. Levels of trans-zeatin and cis-zeatin are varied slightly among wild-type, the OscZOG1-RNAi transgenic line and OscZOG1-overexpression transgenic line