Information on EC 2.4.1.213 - glucosylglycerol-phosphate synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.213
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RECOMMENDED NAME
GeneOntology No.
glucosylglycerol-phosphate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP-alpha-D-glucose + sn-glycerol 3-phosphate = 2-(alpha-D-glucopyranosyl)-sn-glycerol 3-phosphate + ADP
show the reaction diagram
acts with EC 3.1.3.69: glucosylglycerol phosphatase to form glucosylglycerol, an osmolyte that endows cyanobacteria with resistance to salt
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
ADP-alpha-D-glucose:sn-glycerol-3-phosphate 2-alpha-D-glucopyranosyltransferase
Acts with EC 3.1.3.69 (glucosylglycerol phosphatase) to form glucosylglycerol, an osmolyte that endows cyanobacteria with resistance to salt.
CAS REGISTRY NUMBER
COMMENTARY hide
161515-13-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isolated from freshwater lake Chad in Nigeria, gene ggpS
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Manually annotated by BRENDA team
isolated from freshwater lake Chad in Nigeria, gene ggpS
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Manually annotated by BRENDA team
TT-1 and YK-1 strains isolated from freshwater and brackish lakes in Myanmar and Nigeria, gene ggpS
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Manually annotated by BRENDA team
strain AvOP
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Manually annotated by BRENDA team
strain AvOP
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Manually annotated by BRENDA team
a plant associated bacterium, gene ggpPS
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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GgpS is the key enzyme of the glucosylglycerol pathway The intermediate is dephosphorylated by the second enzyme of the pathway, the glucosylglycerol-phosphate phosphatase, GgpP
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-glucose + sn-glycerol 3-phosphate
2-(beta-D-glucosyl)-sn-glycerol 3-phosphate + ADP
show the reaction diagram
UDP-glucose + sn-glycerol 3-phosphate
2-(beta-D-glucosyl)-sn-glycerol 3-phosphate + UDP
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP-glucose + sn-glycerol 3-phosphate
2-(beta-D-glucosyl)-sn-glycerol 3-phosphate + ADP
show the reaction diagram
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Nucleic acids
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GgpS is inhibited by nucleic acids in a sequence- and length-independent manner in vitro and in vivo, it binds DNA, RNA, and heparin via a salt-dependent electrostatic interaction with the negatively charged backbone of the polyanions. Non-competitive inhibition. The inhibition is completely abolished by 20 mM NaCl
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.48
ADP-glucose
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pH 8.0, 30°C
0.71
sn-glycerol 3-phosphate
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pH 8.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4-6°C, 10 days, 40%
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-type and truncated mutant enzymes from Escherichia coli strain LMG194 by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana ecotype Columbia cytoplasm
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expression in Escherichia coli
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gene ggpPS, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and truncated mutant enzymes in Escherichia coli strain LMG194, complementation of the glucosylglycerol-defective mutants of the cyanobacterium Synechocystis sp. strain PCC 6803
gene ggpS, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene ggpS, expression of Strep-tagged GgpS in Synechocystis sp.
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information