Information on EC 2.4.1.209 - cis-p-coumarate glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.209
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RECOMMENDED NAME
GeneOntology No.
cis-p-coumarate glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + cis-p-coumarate = 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
show the reaction diagram
cis-caffeic acid also serves as a glucosyl acceptor with the enzyme from Sphagnum fallax kinggr. The corresponding trans-isomers are not substrates
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:cis-p-coumarate beta-D-glucosyltransferase
cis-Caffeic acid also serves as a glucosyl acceptor with the enzyme from Sphagnum fallax kinggr. The corresponding trans-isomers are not substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
196887-88-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
american beech
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Manually annotated by BRENDA team
peat moss
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cis-caffeic acid + UDP-glucose + H2O
4'-O-beta-D-glucosyl-cis-caffeate + UDP
show the reaction diagram
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enzyme activity of 10% compared with that using cis-p-coumaric acid as substrate
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?
cis-p-coumarate + UDP-glucose + H2O
4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
show the reaction diagram
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?
UDP-glucose + cis-coniferyl alcohol + H2O
?
show the reaction diagram
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?
UDP-glucose + cis-p-coumarate + H2O
4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + cis-coniferyl alcohol + H2O
?
show the reaction diagram
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?
UDP-glucose + cis-p-coumarate + H2O
4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
show the reaction diagram
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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14 mM, 30% inhibition
cis-p-coumaric acid
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inhibits the rection above 1 mM concentration
NaCl
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0.4 M, 40% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.044
cis-p-coumaric acid
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0.11
UDP-glucose
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cosubstrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.3
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100 mM glycine KOH
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9.3
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 9.5
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below pH 7.8 and above pH 9.5 rapid drop in enzyme activity
288689
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, freezing results in a loss of activity of more than 50%
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4°C, loses more than 25% of its activity within 24 h, 5% glycerol causes total loss of activity after 48 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially, 600fold
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