Information on EC 2.4.1.205 - galactogen 6beta-galactosyltransferase

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The expected taxonomic range for this enzyme is: Helix pomatia

EC NUMBER
COMMENTARY hide
2.4.1.205
-
RECOMMENDED NAME
GeneOntology No.
galactogen 6beta-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + galactogen = UDP + (1->6)-beta-D-galactosylgalactogen
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:galactogen 6-beta-D-galactosyltransferase
Galactogen from Helix pomatia is the most effective acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
88273-54-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-galactose + arabinogalactan
UDP + 1,6-beta-D-galactosylarabinogalactan
show the reaction diagram
-
arabinogalactan from larch wood, 22% as effective as galactogen from Helix pomatia
-
-
?
UDP-D-galactose + galactan
UDP + 1,6-beta-D-galactosylgalactan
show the reaction diagram
-
galactan from bovine lung 64% as effective as galactogen from Helix pomatia
-
-
?
UDP-D-galactose + galactogen
UDP + 1,6-beta-D-galactosylgalactogen
show the reaction diagram
-
galactogen from Helix pomatia best acceptor, galactogen from Lymnaea stagnalis, 22% as effective as galactogen from Helix pomatia
-
-
?
UDPgalactose + galactogen
UDP + 1,6-beta-D-galactosylgalactogen
show the reaction diagram
-
biosynthesis of galactogen
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPgalactose + galactogen
UDP + 1,6-beta-D-galactosylgalactogen
show the reaction diagram
-
biosynthesis of galactogen
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
-
10 mM, 30% stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.36
UDP-D-galactose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.466
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.5
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
endomembrane-associated, probably in Golgi complex
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
x * 70000, SDS-PAGE
600000
-
greater than 600000 Da, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 70000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, crude homogenate, 6 months
-
0°C, 24 h, 50% loss of activity
-
4°C, purified enzyme, stable for 1 week
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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