Information on EC 2.4.1.203 - trans-zeatin O-beta-D-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.203
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RECOMMENDED NAME
GeneOntology No.
trans-zeatin O-beta-D-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + trans-zeatin = UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cytokinin-O-glucosides biosynthesis
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Zeatin biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:trans-zeatin O-beta-D-glucosyltransferase
Unlike EC 2.4.1.215, cis-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose can also act as donor (cf. EC 2.4.2.40, zeatin O-beta-D-xylosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
123644-76-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
strain L. cv. Wisconsin, wild type and mutants over-expressing trans-zeatin O-glucosyltransferase gene under 35S or SAG12 promoters
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-
Manually annotated by BRENDA team
no activity in Phaseolus vulgaris
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-
-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
cultivar Cabernet Sauvignon
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
-
-
-
?
UDP-galactose + trans-zeatin
UDP + O-galactosylzeatin
show the reaction diagram
UDP-glucose + m-topolin
UDP + O-beta-D-glucosyl-m-topolin
show the reaction diagram
23% of the activity with trans-zeatin
-
-
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
UDP-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
show the reaction diagram
UDP-xylose + trans-zeatin
UDP + O-xylosylzeatin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required; required; required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
cis-zeatin
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0.14
m-topolin
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0.028 - 0.05
trans-zeatin
0.216
UDP-glucose
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-
2.7
UDP-xylose
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-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
cis-zeatin
Phaseolus lunatus
Q9ZSK5
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0.08
m-topolin
Phaseolus lunatus
Q9ZSK5
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0.45
trans-zeatin
Phaseolus lunatus
Q9ZSK5
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000066
T152F, no activity in wild type
0.00001
CTC151SFS mutant
0.00001166
F149L mutant
0.00001666
F149Y mutant
0.0000216
R59K mutant
0.0000233
L127M mutant
0.0000316
D87N mutant
0.0000533
D87A mutant
0.0000916
D87E mutant
0.000105
L58A mutant
0.00015
R54A mutant
0.000175
D86A mutant
0.000186
E85A mutant
0.0002
wild type
0.000205
Q55A mutant
0.57
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
assay at
30
assay at; assay at; assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immature
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination, expression in Escherichia coli BL21(DE3) cells
expressed in Nicotiana tabacum cultivar Wisconsin 38 leaves
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expression in Escherichia coli
expression in Escherichia coli for sequencing
gene UGT85A1, overexpression of the wild-type enzyme in Arabidopsis thaliana using transfection via Agrobacterium tumefaciens strain GV3101, expression as GFP-tagged enzyme in onion epidermis by particle bombardment
transgenic Zea mays plants are obtained using an Agrobacterium mediated transformation procedure, Ubi: ZOG1 maize line overexpressing the ZOG1 gene of Phaseolus lunatus display increased levels of zeatin-O-glucoside, zeatin O-glucosylation affects root formation, leaf development, chlorophyll content, senescence, male flower differentiation and the formation of tasselseed
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
zeatin O-glucosyltransferase is first upregulated from pre-veraison to veraison, and then downregulated after veraison
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D86A
site-directed mutagenesis
D87A
site-directed mutagenesis
D87E
site-directed mutagenesis
D87N
site-directed mutagenesis
E85A
site-directed mutagenesis
F149A
site-directed mutagenesis
F149L
site-directed mutagenesis
F149Y
site-directed mutagenesis
L127A
site-directed mutagenesis, no activity
L127F
site-directed mutagenesis, no activity
L127M
site-directed mutagenesis
L58A
site-directed mutagenesis
Q55A
site-directed mutagenesis
R54A
site-directed mutagenesis
R59A
site-directed mutagenesis, no activity
R59K
site-directed mutagenesis
R59Q
site-directed mutagenesis, no activity
CTC151SFS
site-directed mutagenesis
T152F
site-directed mutagenesis
additional information
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construction of chimeric recombinant enzyme mutants, exchange of the C-terminus with zeatin O-beta-D-xylosyltransferase, EC 2.4.2.40, gene ZOX1, determination of the site determining the substrate specificity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
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impact of constitutive and senescence-induced ZOG1 over-expression using the 35S and SAG12 promoters on cytokinin levels, indolyl-3-acetic acid and abscisic acid content during the onset and progression of water deficit in tobacco plants and during the early stages of their recovery following rehydration is investigated