Information on EC 2.4.1.194 - 4-hydroxybenzoate 4-O-beta-D-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.194
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RECOMMENDED NAME
GeneOntology No.
4-hydroxybenzoate 4-O-beta-D-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 4-hydroxybenzoate = UDP + 4-(beta-D-glucosyloxy)benzoate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ubiquinone and other terpenoid-quinone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:4-hydroxybenzoate 4-O-beta-D-glucosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
120860-68-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
brassinolide in culture medium decreases enzyme activity
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPglucose + 4-hydroxybenzoate
UDP + 4-(beta-D-glucosyloxy)benzoate
show the reaction diagram
UDPglucose + 4-nitrophenol
UDP + 4-nitrophenyl O-beta-D-glucoside
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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50% increase of activity at 0.3 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-hydroxybenzoic acid
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3-Hydroxybenzoic acid
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competitive inhibition
4-(beta-D-glucosyloxy)benzoate
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non competitive inhibition with respect to both 4-hidroxybenzoic acid and UDP
4-coumaric acid
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4-hydroxybenzoic acid
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Ca2+
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weak effect
Co2+
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strong inhibition at 1 mM and 10 mM
Cu2+
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strong inhibition at 1 mM and 10 mM
EDTA
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negative influence on activity at 10 mM, but reversible
Fe2+
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strong inhibition at 1 mM and 10 mM
gallic acid
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competitive inhibition
Mg2+
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weak effect
Mn2+
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weak effect
Ni2+
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strong inhibition at 1 mM and 10 mM
UDP
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non competitive inhibition with respect to 4-hydroxybenzoic acid, competitive inhibitor with respect to UDPglucose
vanillic acid
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competitive inhibition
Zn2+
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strong inhibition at 1 mM and 10 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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60% increase of activity at 0.1 mM, probably due to removal of heavy metal ions
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.264 - 2.9
4-hydroxybenzoate
0.6
4-nitrophenol
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0.24 - 0.268
UDPglucose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1
2-hydroxybenzoic acid
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0.4
3-Hydroxybenzoic acid
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5
4-coumaric acid
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0.7
gallic acid
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1.3
vanillic acid
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002196
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the activity of p-hydroxylbenzoic acid glucosyltransferase decreases from 0.002586 micromol/min/mg to 0.002196 micromol/min/mg at 16 Gy of gamma radiation. Samples are taken two weeks after radiation.
0.002292
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the activity of p-hydroxylbenzoic acid glucosyltransferase decreases from 0.002586 micromol/min/mg to 0.002292 micromol/min/mg at 2 Gy of gamma radiation. Samples are taken two weeks after radiation.
0.002586
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the activity of p-hydroxylbenzoic acid glucosyltransferase decreases from 0.002586 micromol/min/mg to 0.002292 micromol/min/mg at 2 Gy of gamma radiation. Samples are taken two weeks after radiation.
additional information
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activity is regulated by white light in cell suspension cultures
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 8.6
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half-maximal activity at pH 6.6 and 8.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
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apparent, from gel filtration
47500
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apparent, from gel filtration
51000
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1 * 51000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 51000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithiothreitol stabilizes at 1-10 mM
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phenylmethylsulfonyl fluoride stabilizes at 0.02-1 mM
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.05 M Tris-HCl, 1 mM DTT, 0.02 mM phenylmethylsulfonyl fluoride, pH 7.6, 3 months, 10% loss of activity
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4-8°C, 0.01 M Tris-HCl, pH 7.2, 15 days, 76% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, using ammonium sulfate precipitation and chromatography on DEAE-Sepharose and phenyl-Sepharose
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using gel filtration followed by hydroxyapatite and HiTrap blue affinity chromatography
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