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Information on EC 2.4.1.189 - luteolin 7-O-glucuronosyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Secale cereale
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luteolin 7-O-glucuronosyltransferase
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UDP-glucuronate + luteolin = UDP + luteolin 7-O-beta-D-glucuronide
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hexosyl group transfer
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luteolin triglucuronide biosynthesis
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Flavone and flavonol biosynthesis
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UDP-glucuronate:luteolin 7-O-glucuronosyltransferase
The enzyme participates in the biosynthesis of luteolin triglucuronide, the major flavone found in the photosynthetically-active mesophyll of the primary leaves of Secale cereale (rye).
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glucuronosyltransferase, uridine diphosphoglucuronate-luteolin 7-O-
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rye
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brenda
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UDPgalactose + luteolin
UDP + luteolin 7-O-galactoside
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10% of activity with UDPglucuronic acid
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?
UDPglucose + luteolin
UDP + luteolin 7-O-glucoside
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10% of activity with UDPglucuronic acid
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?
UDPglucuronic acid + apigenin
UDP + apigenin 7-O-glucuronide
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38% of activity compared to luteolin
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UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
UDPxylose + luteolin
UDP + luteolin 7-O-xyloside
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10% of activity with UDPglucuronic acid
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UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
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ir
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
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first enzyme in the biosynthesis of luteolin triglucuronide
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ir
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UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
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first enzyme in the biosynthesis of luteolin triglucuronide
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ir
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Ca2+
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0.25 mM, 25% stimulation
Mg2+
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up to 1 mM, 20% stimulation
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luteolin
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0.14 mM, 60% inhibition
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0.012
UDPglucuronic acid
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6.5
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50% of maximal activity at pH 5.6
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5.6 - 9
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50% of maximal activity at pH 5.6 and pH 9.0
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brenda
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probably
brenda
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no activity in vacuoles
brenda
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freezing without glycerol, complete inactivation
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-20°C, 50% glycerol, 3 months, 30-50% loss of activity
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ultrogel AcA 44, DEAE-cellulose, partial purification
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Schulz, M.; Weissenböck, G.
Three specific UDP-glucuronate: flavone-glucuronosyl-transferases from primary leaves of Secale cereale
Phytochemistry
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1261-1267
1988
Secale cereale
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brenda
Anhalt, S.; Weissenböck, G.
Subcellular localization of luteolin glucuronides and related enzymes in rye mesophyll
Planta
187
83-88
1992
Secale cereale
brenda
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