Information on EC 2.4.1.189 - luteolin 7-O-glucuronosyltransferase

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The expected taxonomic range for this enzyme is: Secale cereale

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EC NUMBER
COMMENTARY hide
2.4.1.189
-
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RECOMMENDED NAME
GeneOntology No.
luteolin 7-O-glucuronosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucuronate + luteolin = UDP + luteolin 7-O-beta-D-glucuronide
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
luteolin triglucuronide biosynthesis
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-
Flavone and flavonol biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucuronate:luteolin 7-O-glucuronosyltransferase
The enzyme participates in the biosynthesis of luteolin triglucuronide, the major flavone found in the photosynthetically-active mesophyll of the primary leaves of Secale cereale (rye).
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CAS REGISTRY NUMBER
COMMENTARY hide
115490-49-8
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPgalactose + luteolin
UDP + luteolin 7-O-galactoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucose + luteolin
UDP + luteolin 7-O-glucoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucuronic acid + apigenin
UDP + apigenin 7-O-glucuronide
show the reaction diagram
-
38% of activity compared to luteolin
-
?
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
UDPxylose + luteolin
UDP + luteolin 7-O-xyloside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
-
first enzyme in the biosynthesis of luteolin triglucuronide
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ir
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
0.25 mM, 25% stimulation
Mg2+
-
up to 1 mM, 20% stimulation
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
luteolin
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0.14 mM, 60% inhibition
UDP
-
strong inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008
luteolin
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-
0.012
UDPglucuronic acid
-
-
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26
UDP
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
50% of maximal activity at pH 5.6
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 9
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50% of maximal activity at pH 5.6 and pH 9.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34500
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gel filtration
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing without glycerol, complete inactivation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, 3 months, 30-50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ultrogel AcA 44, DEAE-cellulose, partial purification
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.189)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)